EMS26096

The Differential Quik III Stain Kit is a high quality, rapid turnaround time staining kit that serves many purposes in the cost-conscious laboratory environment. The Differential Quik III Stain Kit may be used for fine needle aspirations, cytology, blood smears, bone marrow biopsies as well as bone marrow smears, touch preps, various microorgan-isms, as well as detecting the H.Pylori Microorganism. The kit contains a fixative for air-dried cell suspensions and/or touch preparation slides.

Applications:

  • Fine needle aspirations

  • Frozen Sections

  • Blood Smears

  • Cytological specimens

  • Cytopreps

  • Bone marrow biopsy

  • Touch preps

  • Microorganisms

Kit Contents:

  • Fixative - Teal

  • Solution I - Red/Orange

  • Solution II - Blue

Storage and handling:

Storage - Room Temperature
Safety - Refer to the MSDS for details.

Procedure:

  1. Fixative: Fix frozen sections, touch preps, bone marrow biopsies and blood smears for 10 seconds inFixative.

  2. Solution I-Red: Dip 5 times in Solution I

  3. Solution II-Blue: Dip 5 times in Solution II

  4. Wash in Distilled water and air dry.

  5. Quickly dehydrate in 100% EtOH,then xylene and mount in a synthetic resin.

Results:

Structure

Colour

Erthrocytes

Pink/yellowish red

Platelets

Dark blue/violet/purple granules

Neutrophils

Blue nucleus, pink cytoplasm, purple granules

Eosinophils

Blue nucleus, blue cytoplasm, red granules

Basophils

Dark blue purple nucleus, purple granules

Monocytes

Purple nucleus, light blue cytoplasm

Bacteria/microorganisms

Deep blue, vary in shape

Collagen, cytoplasm & muscle

Various shades of pink, blue, orange & yellow