EMS26090

 

Staining Protocol

  1. Deparaffinise and hydrate to distilled water.
  2. Stain in filtered Congo Red, 1% Aqueous for 1 hour.

Note: If using a microwave, stain in Congo Red, 1% Aqueous for 25 seconds. The hot stain jar is removed from the oven and should sit at room temperature for 1 minute.

  1. Rinse in distilled water.
  2. Quickly differentiate in Alkaline Alcohol.
  3. Wash in running tap water for approximately 5 minutes.
  4. Counterstain in Mayer's Acid Haemalum for approximately 5 minutes.

Note: If using a microwave, oven-stain for 30 seconds.

  1. Wash in running tap water for 15 minutes.
  2. Dehydrate and clear through 95% ethyl (absolute alcohol changes each), 2 minutes each.
  3. Mount using resinous medium.

 

Stain Results

Amyloids

Pink Red

Nuclei

Blue

Elastic Tissue

Light Red