EMS26041

Components:

  • Harris Haematoxylin

  • Distilled water

  • Glacial Acetic Acid

  • Acid Alcohol

  • Sodium Acetate, 1% or Ammonia Water, 0.3%, or Lithium Carbonate Solution

Fixation:

Follow most fixatives.

Sections:

Paraffin

Staining:

  1. Deparaffinise and hydrate to distilled water.

  2. Stain in Harris Haematoxylin containing 2 – 4ml of Glacial Acetic Acid per 100ml for 5-15 minutes. Wash in water.

  3. Differentiate in Acid Alcohol until the sections turn red.

  4. Blue section in Sodium Acetate, 1% or Ammonia Water, 0.3%, or Lithium Carbonate Solution

  5. Wash thoroughly and counterstain.

References:

Harris, H.F., J.  Appl. Microscopy, III: 777-781, 1900

Mallory, F. B., Pathological Technique, W. B. Saunders Co., Philadelphia, 1938.

Clark, G., (ed.), Staining Procedures, 3rd Edition, William & Wilkins, Baltimore, pp33 & 35, c 1973.

Luma, L.G., (ed.) Manual of Histologic Staining Method of the AFIP, 3rd edition, McGraw-Hill, NY, pp 34 & 38, c1968.