Van Gieson's Solution

EMS26046

 VAN GIESON’S METHOD FOR COLLAGEN FIBRES

Components:

• Van Gieson's Solution

• 95% alcohol

• Permount

• Weigert's Iron Hematoxylin A

• Weigert's Iron Hematoxylin B

Fixation:

Any

Sections:

Paraffin @ 6µm or Celloidin

Procedure:

1. Deparaffinise and hydrate to distilled water

2. Weigert's Working Haematoxylin* for 10 minutes.

3. Wash in distilled water.

4. Stain 1-3 minutes in Van Gieson's Solution

5. Dehydrate in 95% alcohol, absolute alcohol, two changes each. Clear in two changes xylene.

6. Mount with Permount or equivalents.

*Weigert's Working Solution is prepared by mixing equal parts of Weigert's Iron Hematoxylin A and Weigert's Iron Hematoxylin B

Stain Results:

References:

Clark, G., (ed.) Staining Procedures 3 rd Ed., Williams & Wilkins Co. Baltimore, c. 1973, p.50

Luna, L., (ed.) AFIP Manual of Histological Staining Methods, 3 rd Ed., New York . McGraw Hill Publication, c. 1968, p. 76.

Van Gieson, I. , New York Med. J., 50:57-60, 1889.

Mallory, F.B., Pathological Technique, W.B. Saunders Co., Philadelphia 1938.

VERHOEFF’S ELASTIC TISSUE STAIN

 Components:

• Potassium Iodide, 3%, aqueous

• Alcoholic Haematoxylin, 10%

• Van Gieson's Solution

Fixation:

10% Formalin or Zenker's Stock Solution

Section:

Paraffin @ 6µm

Procedure:

1. Deparaffinise and hydrate to distilled water.

2. Place in staining solution for 15 minutes. Staining solution should be prepared fresh.
   Combine: 25ml Iron solution.
   10ml Potassium Iodide, 3%, aqueous.
   3.5ml Alcoholic Haematoxylin, 10%.
   Use once and discard.

3. Wash in tap water. Differentiate in Iron Solution, diluted 1-4 with distilled water

4. Counter stain with Van Gieson's Solution, 3 - 5 minutes.

5. Dehydrate, clear and mount.

Stain Results:

References:

Clark, G., Ed: Staining Procedures. 3 rd ed., Baltimore : William and Wilkens Co. c. 1972, p. 61

AFIP Manual of Histologic Staining Techniques. 3 rd ed., Ed. G.. Luna: New York : McGraw-Hill Publications, C. 1968, p. 76