EMS26046
VAN GIESON’S METHOD FOR COLLAGEN FIBRES
Fixation:
Any
Sections:
Paraffin @ 6µm or Celloidin
Staining:
- Deparaffinise and hydrate to distilled water
- Weigert's Working Haematoxylin* for 10 minutes.
- Wash in distilled water.
- Stain 1-3 minutes in Van Gieson's Solution (EMS26046-05)
- Dehydrate in 95% alcohol, absolute alcohol, two changes each. Clear in two changes xylene.
- Mount with Permount (EMS17986-01) or equivalents.
*Weigert's Working Solution is prepared by mixing equal parts of Weigert's Iron Hematoxylin A (EMS26044-05) and Weigert's Iron Hematoxylin B (EMS26044-15)
Staining Results:
Collagen: Red
Smooth and striated muscle: Yellowish to Brownish
Cornified epthelium: Yellow
Hyalin: Yellow
Nuclei: Blue to Black
References:
Clark, G., (ed.) Staining Procedures 3 rd Ed., Williams & Wilkins Co. Baltimore, c. 1973, p.50
Luna, L., (ed.) AFIP Manual of Histological Staining Methods, 3 rd Ed., New York . McGraw Hill Publication, c. 1968, p. 76.
Van Gieson, I. , New York Med. J., 50:57-60, 1889.
Mallory, F.B., Pathological Technique, W.B. Saunders Co., Philadelphia 1938.
VERHOEFF’S ELASTIC TISSUE STAIN
Fixation:
10% Formalin or Zenker's Stock Solution (EMS64123)
Section:
Paraffin @ 6µm
Staining Procedure:
- Deparaffinise and hydrate to distilled water.
- Place in staining solution for 15 minutes. Staining solution should be prepared fresh.
Combine: 25ml Iron solution.
10ml Potassium Iodide, 3%, aqueous.
3.5ml Alcoholic Haematoxylin, 10%.
Use once and discard. - Wash in tap water. Differentiate in Iron Solution, diluted 1-4 with distilled water
- Counter stain with Van Gieson's Solution, 3 - 5 minutes.
- Dehydrate, clear and mount.
Results:
Elastic Fibres: Intensely blue-black
Nuclei: Blue to black
Collagen: Red
Smooth and Striated Muscle: Brownish
References:
Clark, G., Ed: Staining Procedures. 3 rd ed., Baltimore : William and Wilkens Co. c. 1972, p. 61
AFIP Manual of Histologic Staining Techniques. 3 rd ed., Ed. G.. Luna: New York : McGraw-Hill Publications, C. 1968, p. 76