EMS26173

Fixation

Formalin-Acetone Fixative

Procedure

  1. Fix thin blood films in Formalin Acetone Fixative for 15 seconds.

  2. Rinse gently in distilled water.

  3. Stain in 3-Amino-9-Ethylcarbazole staining solution for 2.5 minutes.

  4. Wash in running tap water.

  5. Stain in Mayer's Haematoxylin for 8 minutes.

  6. Wash in tap water, air dry, and mount in Glycerine Jelly.

Stain Results

Sites of peroxidase activity

Red-Brown granules

Nucle

Blue