EMS11430

Bismuth Stain Kits for Specific En Bloc Staining Kit consists of:

  • 10 x 5ml Solution A (Sodium Tartrate, NaOH and Bismuth Subnitrate)

  • 10 x 10ml Solution B (0.2M Triethanolamine-HCl buffer, pH 7.0)

Procedure:

  1. Mix Solution A and B together (One part of A into two parts of B) then adjust to pH 7.0 with 1N HCl.

  2. Aldehyde-fixed tissue blocks are rinsed in the above buffer and stained en-bloc with Bismuth for one hour at room temperature on a gentle rotary shaker. Post fixation is carried out with 1% Osmium Tetroxide in 0.05 M Sodium Cacodylate buffer, pH 7.2.

  3. Post-staining is not needed.

Reference:

Locke & Huie, (1977). Bismuth staining for Light and Electron Microscopy. Tissue Cell 9, 347.