EMS11436
The Bismuth Stain Kit for Staining of Mucosubstances and Polysaccharides consists of:
10 x 10ml Solution A (Periodic acid, Ethanol, Sodium acetate in distilled water)
10 x 10ml Solution B (Sodium tartrate, NaOH, Bismuth subnitrate)
Procedure:
Thin sections of conventionally fixed tissues are mounted on copper grids and floated on solution A for 10-30 minutes at room temperature.
After being thoroughly washed 20-30 times for 5-10 minutes with distilled water to remove periodate, the grids are floated on solution B for 30-60 minutes at room temperature. They are then again thoroughly washed with distilled water.
Reference:
Ainsworth, S.K., Ito S., and Karnovsky,M.J. (1972). Alkaline bismuth reagent for high resolution ultrastructural demonstration of periodic-reactive sites. J. Histochem, Cytochem. 20, 995.