EMS15550
Ferritin Cationised Specifications
Quantity: 10mg per ml
Form: Dissolved in 0.15M NaCl sterile solution
Protein Concentration: 1%
10.5 mg/ml based on E 270nm = 79.9 as for native ferritin.
Cationised ferritin is prepared according to the publication of D. Danon et. al. Journal of
Ultrastructure Research 38, 500-510 (1972).
The Nucleophile used for the coupling is DMPA (N,N-dimethyl-1, 3 propanediamine).
Storage: Store at 4°C
Instructions for use:
Labelling of negatively charged cell membranes for visualisation in the electron microscope is carried out by using the cationised ferritin solution (0.1ml), diluted to 0.5ml with Veronal-HCl buffered saline pH 7.2. This working dilution is added to a 10% cell suspension (1ml) before or after a short fixation with glutaraldehyde. Shake the mixture gently and allow to stand at ambient temperature. The optimal incubation for unfixed cells is 5 minutes; longer periods can give rise to toxic effects (the incubation and temperature conditions may be changed according to the requirements of the experiment). Wash the cell suspension with Veronal-HCl buffered saline and prepare for electron microscopy.
Note:
Laboratory reagent - not to be administered to humans nor used for any drug purposes.