EMS26153

Components:

  • Giemsa Thin Film solution

  • Giemsa Stock solution

  • Phosphate Buffer

Fixation:

Streak a drop of blood, leukocytes, rickettsiae culture, pus or other exudates over a sterile glass slide by means of another slide or cover glass. Fix film in Methyl Alcohol for 2-3 minutes.

Staining:

  1. Place in Ethyl or Methyl Alcohol for 5 – 7 minutes. Air dry.

  2. Stain in the Giemsa Thin Film Staining solution*. Because different cells accept the stain at varying rates, prepare 3 – 4 sample slides and stain from 15 – 20 minutes. Some membranous structures (Trypanosomes) may require 1 – 2 hour periods.

  3. To prepare Giemsa Thin Film Staining solution from Giemsa Stock solution, mix before use:
    Giemsa Stock Solution - 1ml
    Phosphate Buffer - 50ml

  4. Wash in distilled water and air dry.

Stain Results:

Nuclei, additional Leukocyte features are similar to Wright Stain results

Reddish Purple

Plasmodic cytoplasm

Blue

Chromatin

Red

References:

Clark, G., Staining Procedures, Williams and Wilkins Co. Baltimore, 3rd, c. 1973, p. 125