EMS26357

This method eliminates cytoplasmic staining, revealing thin septa and collagen fibres clearly.

Components:

  • Phosphomolybdic Acid, 0.2% Aqueous

  • Sirius Red, 0.1% In Saturated Picric Acid

  • Hydrochloric Acid, 0.01N

Fixation:

Bouins' Fixative

Sections:

Parrafin @ 7 microns.

Procedure:

  1. Deparaffinise and hydrate to distilled water.

  2. Wash in running tap water, 10 minutes.

  3. Rinse in distilled water.

  4. Treat in Phosphomolybdic Acid 0.2% Aqueous (1-5 minutes)

  5. Stain in Sirius Red, 0.1% in Sat'd Picric Acid , for 90 minutes.

  6. Wash for 2 minutes in 0.01 N Hydrochloric Acid.

  7. Rinse in 70% Alcohol for 45 seconds.

  8. Dehydrate, clear and mount in Permount

Stain Results:

Septa

Red

References:

Dolber, Paul C. and Madison S. Spach. Picrosirus Red Staining of Cardiac Muscle Following Phosphomolybdic Acid Treatment , Stain Technology , Vol.62 No.1 (1987) 23-26.