EMS26357

This method eliminates cytoplasmic staining, revealing thin septa and collegen fibres clearly.

Components

Phosphomolybdic Acid, 0.2% Aqueous

Sirius Red, 0.1% In Saturated Picric Acid

Hydrochloric Acid, 0.01N

Fixation

Bouins' Fixative

Sections

Parrafin @ 7 microns.

Staining

  1. Deparaffinise and hydrate to distilled water.
  2. Wash in running tap water, 10 minutes.
  3. Rinse in distilled water.
  4. Treat in Phosphomolybdic Acid 0.2% Aqueous (1-5 minutes)
  5. Stain in Sirius Red, 0.1% in Sat'd Picric Acid , for 90 minutes.
  6. Wash for 2 minutes in 0.01 N Hydrochloric Acid.
  7. Rinse in 70% Alcohol for 45 seconds.
  8. Dehydrate, clear and mount in Permount

Results

Septa

Red

References

Dolber, Paul C. and Madison S. Spach. Picrosirus Red Staining of Cardiac Muscle Following Phosphomolybdic Acid Treatment , Stain Technology , Vol.62 No.1 (1987) 23-26.