EMS22407

IBI Blue Platinum Stain Pt4N8H6O24C20

Preparation:

  • Adjust pH of distilled water with concentrated acetic acid to 3.5

  • Dilute PtBlue stock solution as supplied 1:100 in pH-adjusted water

Use:

  • Incubate grid/Epon section on a drop of Pt Blue 1:100 for 5 min at RT

  • Wash on a water drop for about 2 min, repeat 5x

  • Dry grid/section for approx. 10 min in an incubator

  • Stain with lead citrate for 2 min at RT

  • Jet-wash grid 5x (water stream from a plastic pastette)

  • Suck up remaining water with a filter paper and dry thoroughly

Cartilage ECM, contrasted with PtBlue + LC

Cartilage ECM, contrasted with UA + LC

HepG2 cells, contrasted with PtBlue + LC

Depending on the sample, PtBlue can be nearly as good as uranyl acetate in its ability to add contrast. It does not work well with chondrocytes or T. brucei in our hands.

Credits:

Schmidt, Katy. "Technical Note." IBI Labs. Web. 15 May 2015.