EMS26306


Components

Mayer's Haematoxylin

Acid-Fuchsin Aniline Solution

Light Green 1% Aqueous

Phosphomolybdic Acid 1%

Alcoholic Picric Acid


Fixation:

10% Buffered Neutral Formalin or Zenker’s


Sections:

Cut Paraffin, 6µm


Staining Procedures:

  1. Deparaffinize and hydrate to distilled water.
  2. Stain in Mayer Haematoxylin for 15 minutes. Wash in running water for 15 minutes.
  3. Flood slides with Acid Fuchsin-Aniline Solution. Shake bottle well before using, solution will separate upon standing, and heat gently until aniline fumes escapes, view against a dark background. Let stand for 15 minutes. Wash in water, 3 dips.
  4. Differentiate in Alcoholic Picric Acid until only hyaline and red cells remain red, and collagen is faint gray or unstained. Wash in running water for 3 minutes.
  5. Mordant in 1% Phosphomolybdic Acid for 4 to 18 hours.
  6. Counterstain in Light Green Solution for 1 hour. Wash in running water for 1 minute.
  7. Fibers are seen as distinct fibrils.
  8. Dehydrate in 95% alcohol, absolute alcohol, and clear in xylene, two changes each.
  9. Mount with Permount.


Stain Results:

Mallory Bodies

Bright Red

Erythrocytes

Red

Cystoplasm

Pale Brown

Bile Pigment

Green

Protinaceous material seen occasionally in some liver cells

Red

Hemosiderin and lipofichsin

Unstained


References:

Lacquer, G.I., Amer. J. Clin. Path., 20:689-690, 1950.

Luna, L.G. (ed), Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw-Hill, NY, c 1968

Lillies, R.D. Fullmer, H.M., Histopathologic Technic and Pratical Histochemistry 4th ed., McGraw-Hill, N.Y. 1976, pp 670-671.

Clark, G., (ed). Staining Procedures, 3rd edition, Williams & Wilkins, Baltimore, p. 52, c.1973.