EMS26458


Components

Phloxine 1% Aqueous Solution

Phosphotungstic Acid 3% Solution

Azure II Solution, 0.05%

Weigert's Iron Haematoxylin A

Weigert's Iron Haematoxylin B


Fixation

Bouin's without Acetic Acid


Section

Paraffin, @ 6µm


Staining Procedure

  1. Deparaffinise and hydrate to distilled water.
  2. Stain for 10 minutes in Phloxine Solution, 1% Rinse in distilled water.
  3. Place for 1 minute in Phosphotungstic Acid, 3%. Rinse in distilled water.
  4. Stain for 30 seconds in Azure II Solution, 0.05% Rinse in distilled water.
  5. Stain in Weigert's working solution for 1 minute. Rinse in distilled water.
    To prepare Weigert's Working solution:
    Mix just before use:
    1 part Weigert's A
    1 part Weigert's B DO NOT REUSE!!!!!
  6. Dehydrate in 95% alcohol, absolute alcohol and clear in xylene, two changes each.
  7. Mount with Permount


Results


Human Tissue

Alpha granules:

Purple

Beta granules:

Violet Blue

D-cell granules:

Light blue with evident granules

Exocrine cells:

Grayish blue with red granules


References

Maldonado, R. and San Jose, H. Stain Techn., 42:11-13, 1967

Luna , L.G., (ed.), Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw, N.Y.
 p. 105, c. 1968