EMS26458

Components:

  • Phloxine 1% Aqueous Solution

  • Phosphotungstic Acid 3% Solution

  • Azure II Solution, 0.05%

  • Weigert's Iron Haematoxylin A

  • Weigert's Iron Haematoxylin B

Fixation:

Bouin's without Acetic Acid

Section:

Paraffin, @ 6µm

Staining Procedure:

  1. Deparaffinise and hydrate to distilled water.

  2. Stain for 10 minutes in Phloxine Solution, 1% Rinse in distilled water.

  3. Place for 1 minute in Phosphotungstic Acid, 3%. Rinse in distilled water.

  4. Stain for 30 seconds in Azure II Solution, 0.05% Rinse in distilled water.

  5. Stain in Weigert's working solution for 1 minute. Rinse in distilled water.
    To prepare Weigert's Working solution:
    Mix just before use:
    1 part Weigert's A
    1 part Weigert's B DO NOT REUSE!!!!!

  6. Dehydrate in 95% alcohol, absolute alcohol and clear in xylene, two changes each.

  7. Mount with Permount

Stain Results:


Human Tissue

Alpha granules:

Purple

Beta granules:

Violet Blue

D-cell granules:

Light blue with evident granules

Exocrine cells:

Grayish blue with red granules

References:

Maldonado, R. and San Jose, H. Stain Techn., 42:11-13, 1967

Luna , L.G., (ed.), Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw, N.Y.
 p. 105, c. 1968