EMS26369

Components:

  • Alcoholic Haematoxylin

  • Ferric Chloride 2% aqueous

  • Lugol’s Iodine

  • Ferric Chloride 0.4% aqueous

  • Van Geison's Solution

Consistent and shorter staining time!

Fixation:

10% Buffered Neutral Formalin

Sections:

4-5µm

Staining:

  1. Deparaffinise and hydrate sections.

  2. Stain in modified Verhoeff at room temperature for 7 minutes or in a 60°C oven for 3.5 minutes.
    To prepare modified Verhoeff Working Elastic solution, mix just before use:
    • 3 parts Alcoholic Haematoxylin, 3%
    • 2 parts Ferric Chloride 2%, aqueous
    •  1 part Lugol's Iodine

  3. Wash in running warm tap water for 1 minute.

  4. Differentiate in Ferric Chloride, 0.4% aq. for 75 seconds.

  5. Wash in running warm tap water for 5 minutes.

  6. Counterstain in Van Geisons solution for 60 seconds.

  7. Dehydrate, clear and mount with Permount.

Stain Results:

Nuclei

Greyish-black

Elastic Fibres

Bluish-black

Collagen fibres

Red

Muscle

Greenish yellow

References:

Garvey, Winsome et al "A modified Verhoff Elastic Van Geison Stain" The Journal of Histotechnology, Vol. 14, No. 2 pp113-114 June 1991.