Weigert's Resorcin Fuchsin

EMS26370

Components:

Resorcin-Fuchsin Solution

Van Gieson's Solution

Weigert's Iron Haematoxylin A

Weigert's Iron Haematoxylin B

Acid Alcohol 1%

Fixation: 

Any common reagent may be used.

Sections:

Paraffin at 6µm.

Staining Procedures:

1. Deparaffinise and hydrate to distilled water.
2. Stain in Resorcin-Fuchsin Solution for 1 – 12 hours depending upon the depth of stain required for the elastic fibres. (Nuclei may be stained in Weigert Iron Hematoxylin Working Solution* prior to the Resorcin Fuchsin rather than alter as is described here).
   1. * To prepare working solution, (prepare fresh just before use) mix equal parts of Weigert’s Iron Haematoxylin A and Weigert’s Iron Haematoxylin B.
3. Clear the stain in 95% Alcohol. Acid Alcohol may be used to differentiate any slide that has stained diffusely wash in tap water.
4. Stain slides in Waeigert’s Iron Haematoxylin Working Solution (see above) and then counterstain in Van Gieson’s Solution
5. Rinse in distilled water to remove excess Van Gieson’s Stain from slides.
6. Differentiate and hydrate the slides in two changes each of 95% Alcohol and absolute alcohol. Clear the slides in two changes of Xylene.
7. Mount.

Stain Results:

References:

Mallory, F.B.: Pathological Technique; New York, Hafner Publishing Co., c. 1961, p. 168

Clark, G.: Staining Procedures: Baltimore: Williams and Wikins Co., 3rd ed., c. 1973, p. 60

Luna, L.G.: AFIP Histologic Staining Methods: New York: McGraw-Hill Book Co., 3rd ed., c. 1968, p.80.