EMS26511

Components:

  • Nile Blue A Staining Solution

  • Sulphuric Acid 1% Aqueous

Fixation:

Formalin fixatives

Application:

Show differentiation of melanins and lipofuscins

Procedure:

  1. Deparaffinise and hydrate to distilled water.

  2. Stain 20 minutes in Nile Blue A Staining solution.

  3. Rinse quickly with sulphuric acid, 1%.

  4. Dehydrate immediately in four changes of acetone, 15 seconds each. Rinse in Xylene-acetone. Clear in two changes of Xylene.

  5. Mount.

Stain Results:

Lipofuscins

Unstained - natural yellow

Melanins

Green

References:

Staining Procedures, G. Clark: Williams and Wilkins Co., Baltimore, 3 rd ed., c. 1973, p. 122.

Lillie, R.D.: Stain Techn ., 31:151.