EMS26472

Components:

  • Giemsa Stain Solution

  • Acetic Acid, O.5%, Aqueous

  • Pollacks’s Trichrome Solution

Fixation:

10% Buffered Neutral Formalin

Section:

Paraffin, @ 6µm

Procedure:

  1. Deparaffinise and hydrate to distilled water.

  2. Stain in *Working Giemsa Solution for 75 minutes. Rinse in distilled water.
    *Prepare Working Giemsa Solution by adding 40 drops of Giemsa Stock Solution to 40ml of distilled water and mixing thoroughly. Prepare fresh, immediately before use.

  3. Place in Acetic Acid 0.5% for 5 to 10 seconds. Rinse in distilled water.

  4. Stain in Pollack's Trichrome Solution for 10 to 15 seconds.

  5. Rinse in Acetic Acid 0.5% until no more stain washes off, 3 to 5 seconds.

  6. Dehydrate in 95% Alcohol, Absolute Alcohol and clear in Xylene.

  7. Mount with Permount.

Stain Results:

Mast Cell Granules

Deep blue

Collagen & Cartilage

Blue green

Muscle & Elastic Fibres

Red

Fibrin

Purple

Bone

Green

Colloid, Keratin & Erythrocytes

Orange red to orange

References:

Toren, D.A.: Stain Techn ., 38:249-250, 1963.

Luna, L., (ed.), Manual of Histologic Staining Methods of the AFIP , 3 rd . ed.,
 McGraw-Hill , New York , c. 1968, pp. 116-117.