EMS26611

Components:

  • Fouchets Reagent

  • Van Gieson's Solution

Fixation:

10% Buffered Neutral Formalin

Sections:

Paraffin @ 6µm

Staining Procedures:

  1. Deparaffinise and hydrate to distilled water.

  2. Place in Fouchet’s Reagent for 5 minutes. Wash in running water, then in distilled water.

  3. Stain for 5 minutes in Van Gieson’s Solution.

  4. Dehydrate in 95% Alcohol, absolute alcohol, and clear in xylene, two changes each.

  5. Mount with Permount.

Stain Results:  

Biliverdin

Green

Collagen

Red

Muscle

Yellow

References:

Hall, M.J., Amer. J. Clin. Path., 34:313-316, 1960.

Luna, L.G. (ed). Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology, 3rd ed., McGraw-Hill, N.Y., c. 1968, p.174.