EMS26604


Components:

Potassium Ferrocyanide

Potassium Ferricyanide

Hydrochloric Acid 0.5%

Basic Fuchsin 0.5%

Acetic Acid 1% Aqueous


Fixation:

10% Buffered Neutral Formalin


Sections:

Paraffin @ 6µm


Staining Procedures:

(Use control slide. Use chemically clean glassware)


a) Preparation of Potassium Ferrocyanide Solution:

Prepare fresh, just before use

  • Potassium Ferrocyanide - 0.4g
  • Hydrochloric Acid - 40.0ml


b) Preparation of Potassium Ferricyanide Solution:

Prepare fresh just before use.

  • Potassium Ferricyanide - 0.4g
  • Hydrochloric Acid - 40.0ml
  1. Deparaffinise and hydrate to distilled water.
  2. For Ferric Iron, place in Potassium Ferrocyanide Solution for 1 hour. For Ferrous Iron, place in Potassium Ferricyanide Solution for I hour.
  3. Wash in Acetic Acid, 1%.
  4. Stain for 10 minutes in Basic Fuchsin Solution. Rinse in distilled water, (For critical work, omit this step).
  5. Dehydrate in 95% alcohol, absolute alcohol and clear in xylene, two changes each.
  6. Mount with Permount.


Stain Results:

Ferric Iron

Dark Prussian’ Blue

Ferrous Iron

Dark Turnbull’s Blue

Background

Light Red


References:

Lillie, R.D., Histopathologic Technic and Practical Histochemistry, 3rd ed. McGraw-Hill, N.Y., p.407, c 1965.

Luna, L.G. (ed). Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology, 3rd ed., McGraw-Hill, N.Y., c. 1968, p.182.