EMS26087

Components:

  • Kinyoun's Solution

  • Acid Alcohol, 1%

  • Methylene Blue Working Solution

Section:

Paraffin and frozen sections, air dried smears, touch preps.

Procedure:

  1. Deparaffinise sections in 2 changes of Xylene, and absolute alcohol. Air dry slides.

  2. Filter 40ml Kinyoun's Carbol Fuchsin Solution, into a plastic Coplin jar. Place slides in the jar; cover with a loose screw cap. Heat in the microwave for 50 seconds.

  3. Remove the Coplin jar from the oven; rinse slides individually in 50% Alcohol. Differentiate in Acid Alcohol, 1%, until sections are faint pink. Dip a few times. Rinse in 50% alcohol, then in distilled water.

  4. Counterstain lightly with Methylene Blue Working Solution, for two seconds. To prepare working solution from Methylene Blue Stock Solution, mix:
    Methylene Blue Stock -10 parts
     Tap Water - 90 parts

  5. Dehydrate clear in Xylene. Mount

Note: All the alcohol dilutions used in this method should be prepared with absolute 100% alcohol.

Stain Results:

Acid Fast Bacteria

Red

Other tissue elements

Blue

Other types of bacteria

Blue

References:

Valle, Sylvia; Journal of Histology , vol. 9, No. 4, December 1986.