EMS26328
Components:
Aldehyde-Fuchsin Solution pH 1.0
Metanil Yellow Solution 0.25%
Alcohol Solution, 80% pH 1.0
Fixation:
10% Buffered Neutral Formalin or fixative of choice.
Sections:
Paraffin at 6µm sections
Staining Procedures:
- Deparaffinise and hydrate to distilled water.
- Rinse in several changes of Alcohol, 80% pH 1.0.
- Stain in Aldehyde Fuchsin Solution for 30 minutes.
- Rinse off excess stain with Alcohol, 80% pH 1.0.
- Counterstain with Metanil Yellow Solution until background is a light yellow (approximately 1 minute). Rinse in distilled water.
- Dehydrate in 95% alcohol, absolute alcohol, and clear in Xylene, two changes each.
- Mount with Permount or DPX.
Stain Results:
All mucins except the highly acidic sulphated mucosubstances are aldehyde fuchsin negative at pH values of 1.0 and below.
References:
Johnson, W.C., Graham, J.H., and Helwig, E.B., J. Invest. Derm., 42:215-224, 1964.
Luna, L.G., (ed), Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw-Hill, c 1968, p.166