EMS26321


Fixation:

Absolute Alcohol 4 – 8 hours.


Sections:

Paraffin or Celloidin


Staining Procedures:

  1. Deparaffinise and hydrate to distilled water.
  2. Place on staining rack and rinse several times with distilled water.
  3. Place 2 ml of Mayer Mucihematein, Modified on each slide. Allow to remain for five minutes. Drain off stain and wash well with distilled water three times for 5 minutes each.
  4. Dehydrate in two changes of 95% alcohol, two changes of absolute alcohol, Absolute Alcohol-Xylene (50:50), and two changes of Xylene.
  5. Mount with Permount or DPX.


Stain Results:

Mucin

Deep Violet

Cell Nuclei

Pale Grey-Blue

Connective Tissue

Pale Grey to Colourless


References:

Laskey, Alice: Stain Techn., 25:33 (1950).

Clark G.: Staining Procedures, William & Wilkins Company, Baltimore, 3rd Ed., c. 1973, p. 47