EMS26381


Components:

Ferric Ammonium Sulphate 5%

Mayer's Haematoxylin

Picric Acid-Orange G Solution

Ponceau Acid Fuchsin Solution

Phosphotungstic Acid 1% Aqueous

Aniline Blue Solution


Fixation:

Formalin-Saline for 48 hours or more


Sections:

Cut paraffin at 5µm sections


Staining Procedures:

  1. Hydrate slides to distilled water.
  2. Mordant in Ferric Ammonium Sulphate, 5% for 5 minutes. Rinse in running tap water.
  3. Stain with Mayer's Haematoxylin for 5 minutes. Rinse in running tap water.
  4. Stain in the Picric Acid-Orange G Solution for 30 minutes to 1 hour. Rinse once in distilled water.
  5. Stain in Ponceau Fuchsin Solution for 1 to 5 minutes. Rinse in distilled water.
  6. Differentiate with Phosphotungstic Acid, 1% until the muscle is red and background pale pink. Rinse in distilled water.
  7. Stain with Aniline Blue Solution for 30 minutes to 2 hours. Rinse in several changes of distilled water.
  8. Dehydrate, clear in Xylene and coverslip, using a synthetic mounting medium.


Stain Results:

Cell

Fixation Time:

48 Hours or more

Fixation Time:

Less than 48 hours

Fibrin

Bright Red

Orange to Orange Red

Platelets

Grey Blue or Navy

Light Grey

Collagen

Bright Blue

Bright Blue

Muscle

Red

Red

Red Blood Cells

Clear Yellow

Red, Green or Yellow