EMS26671


Components

Cresyl Violet Acetate 2% Aqueous

Acetate Buffer

Cresyl Violet Acetate Working Solution


Fixation

Formalin, 10% Neutral Buffered


Sections

Paraffin @ 6µm


Staining

  1. Deparaffinise and carry through absolute alcohol, two changes.
  2. Let stand in absolute alcohol for two hours.
  3. Stain in Cresyl Violet Acetate Working Solution for 40 – 60 minutes.
  4. Differentiate rapidly in 95% alcohol, then dehydrate in absolute alcohol, and clear in xylene, two changes each.
  5. Mount with Permount.


Staining Results

Nissl Substances

Intense Purple

Nuclei

Purple

Background

Clear


References

AFIP Manual of Histologic Staining Technique; ed. L. Luna., 3rd ed. McGraw-Hill Publications, c. 1968, p. 212.