EMS26671

Components

Cresyl Violet Acetate 2% Aqueous

Acetate Buffer

Cresyl Violet Acetate Working Solution

Fixation

Formalin, 10% Neutral Buffered

Sections

Paraffin @ 6µm

Staining

  1. Deparaffinise and carry through absolute alcohol, two changes.
  2. Let stand in absolute alcohol for two hours.
  3. Stain in Cresyl Violet Acetate Working Solution for 40 – 60 minutes.
  4. Differentiate rapidly in 95% alcohol, then dehydrate in absolute alcohol, and clear in xylene, two changes each.
  5. Mount with Permount.

Staining Results

Nissl Substances

Intense Purple

Nuclei

Purple

Background

Clear

References

AFIP Manual of Histologic Staining Technique; ed. L. Luna., 3rd ed. McGraw-Hill Publications, c. 1968, p. 212.