EMS26671

Components:

  • Cresyl Violet Acetate 2% Aqueous

  • Acetate Buffer

  • Cresyl Violet Acetate Working Solution

Fixation:

Formalin, 10% Neutral Buffered

Sections:

Paraffin @ 6µm

Procedure:

  1. Deparaffinise and carry through absolute alcohol, two changes.

  2. Let stand in absolute alcohol for two hours.

  3. Stain in Cresyl Violet Acetate Working Solution for 40 – 60 minutes.

  4. Differentiate rapidly in 95% alcohol, then dehydrate in absolute alcohol, and clear in xylene, two changes each.

  5. Mount with Permount.

Stain Results:

Nissl Substances

Intense Purple

Nuclei

Purple

Background

Clear

References:

AFIP Manual of Histologic Staining Technique; ed. L. Luna., 3rd ed. McGraw-Hill Publications, c. 1968, p. 212.