Modified Movat's Stain

EMS26385

Components:

• Alcian Blue, 1%

• Alkaline Alcohol

• Orcein, 0.2%

• Haematoxylin Alcoholic, 5%

• Ferric Chloride, 10%

• Lugol’s Iodine

• Woodstain Scarlet-Acid Fuchsin Working Solution

• Acetic Acid, 0.5%

• Phosphotungstic Acid, 5%

• Alcoholic Saffron

Fixation:

10% Buffered Neutral Formalin or Bouin's Solution

Note: If formalin fixative is used, decerated tissue sections must be mordanted in Bouin's Fluid for 1 hour in a 50°C oven. Wash well in running water to remove picric acid deposits.

Sections:

4-6µm

Staining Procedure:

1. Decerate section and hydrate to distilled water.

2. Stain in Alcian Blue, 1% for 20 minutes.

3. Dip five times in distilled water.

4. Place in Alkaline Alcohol in a 56°C oven for 10 minutes.

5. Wash in running tap water for 2 minutes.

6. Stain in Orcein-Verhoeff working solution for 2 hours.
   To prepare Orcein-Verhoeff working solution:
   Immediately before use, in the following order, combine the solutions below:

7. Wash in running tap water for 3 minutes.

8. Stain in Woodstain Scarlet- Acid Fuchsin working solution for 2 1/2 minutes.

9. Place in Acetic Acid, 0.5% for 30 seconds.

10. Differentiate in Phosphotungstic Acid, 5% for 5-10 minutes, well differentiated sections demonstrate colourless collagen and blue-green mucopolysaccharides.

11. Rinse in Acetic Acid, 0.5% for 30 seconds.

12. Three changes of 100% ethyl alcohol, 1 minute each,

13. Stain in Alcoholic Saffron for 8 minutes.

14. Dehydrate in 100% ethyl alcohol, two changes.

15. Clear in xylene, three changes.

16. Coverslip.

Stain Results:

References:

Movat, H.Z.,: Demonstration of all Connective Tissue Elements in a Single Section.
Arch Path., 60:289-295, 1955.

Roman N., et al: Orcein-Hematoxylin in Iodized Ferric Chloride as a Stain for Elastic Tissue.
Stain Techn., 42:199-202, 1967.

Silverman Jefferey., A Modified Movat Pentachrome Stain. Histologic , p. 33. 1972