Modified Movat's Stain

EMS26385

Components

Alcian Blue, 1%

Alkaline Alcohol

Orcein, 0.2%

Haematoxylin Alcoholic, 5%

Ferric Chloride, 10%

Lugol’s Iodine

Woodstain Scarlet-Acid Fuchsin Working Solution

Acetic Acid, 0.5%

Phosphotungstic Acid, 5%

Alcoholic Saffron

Fixation

10% Buffered Neutral Formalin or Bouin's Solution

Note: If formalin fixative is used, decerated tissue sections must be mordanted in Bouin's Fluid for 1 hour in a 50°C oven. Wash well in running water to remove picric acid deposits.

Sections

4-6µm

Staining Procedure

1. Decerate section and hydrate to distilled water.
2. Stain in Alcian Blue, 1% for 20 minutes.
3. Dip five times in distilled water.
4. Place in Alkaline Alcohol in a 56°C oven for 10 minutes.
5. Wash in running tap water for 2 minutes.
6. Stain in Orcein-Verhoeff working solution for 2 hours.
   To prepare Orcein-Verhoeff working solution:
   Immediately before use, in the following order, combine the solutions below:

7. Wash in running tap water for 3 minutes.
8. Stain in Woodstain Scarlet- Acid Fuchsin working solution for 2 1/2 minutes.
9. Place in Acetic Acid, 0.5% for 30 seconds.
10. Differentiate in Phosphotungstic Acid, 5% for 5-10 minutes, well differentiated sections demonstrate colourless collagen and blue-green mucopolysaccharides.
11. Rinse in Acetic Acid, 0.5% for 30 seconds.
12. Three changes of 100% ethyl alcohol, 1 minute each,
13. Stain in Alcoholic Saffron for 8 minutes.
14. Dehydrate in 100% ethyl alcohol, two changes.
15. Clear in xylene, three changes.
16. Coverslip.

Stain Results

References

Movat, H.Z.,: Demonstration of all Connective Tissue Elements in a Single Section.
Arch Path., 60:289-295, 1955.

Roman N., et al: Orcein-Hematoxylin in Iodized Ferric Chloride as a Stain for Elastic Tissue.
Stain Techn., 42:199-202, 1967.

Silverman Jefferey., A Modified Movat Pentachrome Stain. Histologic , p. 33. 1972