EMS26609

Components:

  • Oil Red O Solution 0.5%

  • Propylene Glycol 85%  Aqueous

  • Mayer’s Haematoxylin

Fixation:

10% Buffered Neutral Formalin

Section:

Paraffin at 6µm

Staining Procedure:

  1. Deparaffinise and hydrate to distilled water.

  2. Stain in Oil Red O Solution for 72 hours.

  3. Agitate the slide in Propylene Glycol, 85% for 1 minute.

  4. Two changes of distilled water, 2 minutes each

  5. Stain in Mayer's Haematoxylin for 5 minutes. Wash in running water for 10 minutes.

  6. Mount with Glycerin Jelly

Stain Results:

Lipofuchsin

Red

Nuclei

Blue

References:

Luna, L.G. (ed.), Manual of Histologic Staining Methods of the AFIP , 3 rd edition, The AFIP, 3 rd edition, McGraw-Hill, NY, P. 186, c 1968.