EMS26110

Components:

  • Methanol

  • Giemsa Stock Solution

  • Acetic Acid 1%

  • Fixation

  • 10% Buffered Neutral Formalin

Sections:

3µm

Procedure:

  1. Deparaffinise and hydrate to distilled water.

  2. Place promptly in Methanol for 2 minutes.

  3. Preheat Working Geimsa solution in microwave on high for 45 seconds. Filter the hot solution into a coplin jar containing the slides. Stain for 2 minutes.
    To prepare working solution mix:
    • 7.0ml Geimsa Stock
    • 38ml Distilled water
    • 5ml Methanol
     Prepare fresh- do not reuse!!

  4. Rinse quickly in distilled water

  5. Dip one slide at a time in Acetic Acid 1% .

  6. Rinse quickly in distilled water.

  7. Rinse in 3 changes of Methanol.

  8. Clear in Xylene and mount.

Stain Results:

Red Blood Cells

Pink

Nuclei

Blue

H-Pylori, fungi and other bacteria

Blue

Mast Cells

Purple

Rickettsia

Purple

NOTE: The use of methanol to dehydrate stained sections prior to mounting seems to provide lighter staining of surrounding tissue, offering better contrast with stained organisms.

References:

Helicobacter pylori in peptic ulcer disease, NIH Consensus Statement Online, January 1994, 12 (1): 1:23 . Luna l AFIP 3 rd ed. McGraw Hill, 236.

Fail, Rena , AS , HT (ASCP) Medical University of South Carolina , Histologic . Vol. XXXIV, No. 2, Nov. 2001. P 32.