EMS26114


Components

Jenner’s Stain Stock Solution

Giemsa Stock Solution

Acetic Acid 0.1% Solution


Fixation

10% Buffered Neutral Formalin


Sections

3µm


Procedure

  1. Deparaffinise and hydrate to distilled water.
  2. Place promptly in methanol (methyl alcohol), two changes, three times each.
  3. Stain in Jenner's Working solution for 6 minutes.
    To prepare working solution mix:
    • 1 part Jenner's Stain
    • 1 part Distilled water
     Prepare fresh!
  4. Stain in Working Giemsa solution for 10 minutes.
    To prepare working solution mix:
    • 2.4ml Giemsa Stock
    • 50ml Distilled water
     Prepare fresh-do not reuse!
  5. Differentiate in Acetic Acid, 0.1% (A-114-3) one slide at a time, gently agitate, checking often under microscope until defined.
     DO NOT OVER DIFFERENTIATE!
  6. Dehydrate quickly in tert-Butyl Alcohol to Xylene
  7. Mount.


Stain Results

H-pylori

Blue

Background

Light purple

 

References

Carson, F. Histotechnology: A Self-Instructional Text . 1980-109.