EMS26114

Components:

  • Jenner’s Stain Stock Solution

  • Giemsa Stock Solution

  • Acetic Acid 0.1% Solution

Fixation:

10% Buffered Neutral Formalin

Sections:

3µm

Staining Procedure:

  1. Deparaffinise and hydrate to distilled water.

  2. Place promptly in methanol (methyl alcohol), two changes, three times each.

  3. Stain in Jenner's Working solution for 6 minutes.
    To prepare working solution mix:
    1 part Jenner's Stain
    1 part Distilled water
    Prepare fresh!

  4. Stain in Working Giemsa solution for 10 minutes.
    To prepare working solution mix:
    2.4ml Giemsa Stock
    50ml Distilled water
    Prepare fresh-do not reuse!

  5. Differentiate in Acetic Acid, 0.1% (A-114-3) one slide at a time, gently agitate, checking often under microscope until defined. DO NOT OVER DIFFERENTIATE!

  6. Dehydrate quickly in tert-Butyl Alcohol to Xylene

  7. Mount.

Stain Results:

H-pylori

Blue

Background

Light purple

References:

Carson, F. Histotechnology: A Self-Instructional Text . 1980-109.