EMS26504

Components:

  • Oil Red O Solution

  • Mayer's Haematoxylin

  • Harris' Haematoxylin

  • Propylene Glycol 85% Aqueous Solution

  • Hydrochloric Acid 5%

  • Ammonia Water 0.3%

Fixation:    

10% Buffered Neutral Formalin

Section:      

Frozen

Staining Procedure:

  1.  Cut frozen section and collect in distilled water.

  2.  Propylene Glycol, Absolute, for 2 minutes

  3.  Oil Red O Solution, for one hour. If sections are mounted on glass slides before staining, allow staining process to continue overnight.

  4. Differentiate in Propylene Glycol, 85%, for one minute. Rinse in two changes of distilled water.

  5. Stain for a few seconds in Mayer’s Haematoxylin, or Harris’s Haematoxylin. Rinse in two changes of distilled water.

  6. If over-stained differentiate in Hydrochloric Acid, 5%, wash in water; neutralise in Ammonia Water, 0.3%.

  7. Wash in two changes of distilled water. Mount with Glycerin Jelly.

Stain Results:

Fat

Red

Nuclei

Blue

References:

 AFIP Manual of Histologic Staining Methods, Ed. L. Luna, 3rd ed.,

 New York, McGraw-hill Publications, c. 1968, p. 140.