EMS26504


Components:

Oil Red O Solution
Mayer's Haematoxylin
Harris' Haematoxylin
Propylene Glycol 85% Aqueous Solution
Hydrochloric Acid 5%
Ammonia Water 0.3%


Fixation:    

10% Buffered Neutral Formalin


Section:      

Frozen

 

Staining Procedure:

 

  1.  Cut frozen section and collect in distilled water.
  2.  Propylene Glycol, Absolute, for 2 minutes
  3.  Oil Red O Solution, for one hour. If sections are mounted on glass slides before staining, allow staining process to continue overnight.
  4. Differentiate in Propylene Glycol, 85%, for one minute. Rinse in two changes of distilled water.
  5. Stain for a few seconds in Mayer’s Haematoxylin, or Harris’s Haematoxylin. Rinse in two changes of distilled water.
  6. If over-stained differentiate in Hydrochloric Acid, 5%, wash in water; neutralise in Ammonia Water, 0.3%.
  7. Wash in two changes of distilled water. Mount with Glycerin Jelly.

 

Results:

 

              Fat = Red

              Nuclei = Blue

 

References:

 

 AFIP Manual of Histologic Staining Methods, Ed. L. Luna, 3rd ed.,

 New York, McGraw-hill Publications, c. 1968, p. 140.