Equipment
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Cryo-SEM Preparation System
The PP3010T is a highly automated, easy to use, column‐mounted, gas‐cooled cryo preparation system suitable for most makes and models of SEM, FE‐SEM and FIB/SEM. The PP3010T has all the facilities needed to rapidly freeze, process and transfer specimens. The aQuilo cryo preparation chamber is turbomolecular pumped and includes tools for cold fracturing, controlled automatic sublimation and sputter coating. After processing, the specimen is transferred from the cryo preparation chamber onto a highly stable SEM cold stage for observation. Cold trapping in the cryo preparation chamber and SEM chamber ensures the whole process is frost free. Specimen process times are typically between five and ten minutes. Key features High resolution performance on SEM, FE‐SEM and FIB/SEMs aQuilo gas cooled cryo preparation chamber Efficient cooling (typically down to at least‐190°C) Up to 24 hours run times on one fill of LN2 ‐ allowing unattended overnight operation Large recipe driven touch‐screen interface Automated sublimation, coating and system start up Superb specimen visibility (including preparation chamber CCD camera) Fully compatible with SEM beam deceleration/stage bias modes up to 5kV Off column cooling and pumping ‐ minimum mass on the SEM On‐screen data logging and diagnostics Vacuum storage of the cryo transfer device Prepdek™ workstation ‐ self contained work area, extra bench space not required Specialist support and three year warranty aQuilo cryo preparation chamber Subject to microscope geometry, fits onto a single microscope chamber port ‐ compact installation, frees up microscope ports Mounted directly on the microscope ‐ essential for frost‐free transfer and ease of use Integrated valves ‐ enhanced vacuum performance Gas cooled cold stage and cold traps ‐ rapid cooling down to ‐190°C or lower Turbo pumping system located off‐column ‐ less mass on the SEM Typical vacuum when cold: 10‐6mbar or better ‐ specimen transfer into the SEM always high vacuum to high vacuum Unsurpassed specimen visibility ‐ large front window, top viewing ports and multiple LED chamber lighting Preparation chamber CCD camera for specimen observation ‐ bulky binocular no longer needed Automated high resolution sputter coating using Quorum's market leading "Q" series technology Fracturing/specimen manipulation device Product Description The PP3010T is the very latest in cryo‐SEM technology and combines the highest quality results with unparalleled ease of use. Freezing and specimen handling The PP3010T Prepdek™ workstation is fitted with a slushy nitrogen freezing station, connected to the pumping system. Rapid freezing reduces ice crystal damage and results in improved specimen preservation. For handling pre‐frozen material the Prepdek™ freezing system allows specimens that have been frozen by alternative methods, such as high pressure freezing (or stored field specimens) to be manipulated ‐ under or just above liquid nitrogen ‐ and then vacuum transferred into the aQuilo preparation chamber for subsequent processing and observation. Specimen shuttles and stubs The PP3010T is supplied with universal 10mm specimen stubs with surface slots, holes and a flat area on the reverse side ‐ useful for most specimen types. Holes and slots can be used for liquids and to hold solid material for cross‐section fracturing. Blank and slotted stubs are also included. In addition a range of optional holders is available, including shuttles for large specimens and top‐loading holders for high pressure freezing rivets and planchettes. You can view examples of these by downloading the PP3010T specimen shuttles and stubs PDF at the bottom of this page. Cryo transfer device ‐ including vacuum storage The vacuum transfer device is compact, reliably vacuum tight and has a convenient bayonet connection to the specimen shuttle to ensure rapid pick up and transfer. Set into the Prepdek™ work surface is a pumped storage tube for the cryo transfer device (see Prepdek™ workstation section below). Technical note: Advantages of a SEM mounted cryo preparation chamber The PP3010T aQuilo preparation chamber conveniently combines the advantages of an 'on‐column' and 'off‐column' cryo preparation system. The aQuilo chamber is directly attached to the SEM, but with the turbomolecular pumping and advanced cooling system mounted remotely. In this way the mass and volume attached directly to SEM is kept to a minimum. There are significant advantages of direct connection to the SEM. In particular, specimen transfer is always from high vacuum to high vacuum, which greatly reduces the risk of specimen contamination (frosting). In addition, it makes operation easier and allows the operator a more flexible approach to specimen preparation and observation. For example, during a typical process run it may be advantageous to move the specimen between the preparation chamber and the SEM on a number of occasions, ‐eg to fracture deeper into a specimen, to recoat or simply to first observe the freshly frozen specimen in the SEM for an initial "look‐see", prior to retracting back into the preparation chamber for processing. The aQuilo cryo preparation chamber is connected directly to the SEM and includes a highly efficient nitrogen gas cold stage, extensive cold trapping (above and below the specimen) and facilities to fracture, sublimate and sputter coat specimens. The chamber is fitted with two fully integrated and interlocked gate valves. The outer load‐lock valve includes a pumped airlock which accepts the cryo transfer device; the inner SEM valve ensures rapid high‐vacuum to high‐vacuum specimen exchange. Efficient gas cooled stage and cold traps At the heart of the aQuilo cryo preparation chamber is the nitrogen gas cooled specimen stage. The stage has a dovetail fitting to accept a cryo shuttle and specimen and can be precisely controlled over a temperature range from 100°C to ‐190°C or lower. Large gas cooled cold traps located above and below the specimen stage ensure clean, high vacuum conditions in the chamber. Both the cold stage and cold traps are cooled with the fully integrated CHE3010 off‐column cooling system (see below), which give typical hold times of up to 24 hours between fills. High visibility ‐ plus CCD camera The aQuilo chamber has superb chamber visibility. In addition to the large front window (75 x 150mm) there are two top viewing ports. The chamber is lit by three LEDS and a CCD camera allows the specimen cold stage area to be viewed on the control screen. Images can be saved and stored using a button on the control screen. Cold fracturing Twin fracturing tools (actively cooled) are available and allow a range of specimen types to be cold fractured. The PP33010T is fitted as standard with a front mounted fracturing/manipulation device. The ball‐jointed mount offers flexible movement of the blade and allows the no.5 scalpel to be used both as a surface pick (probe) and a fracturing knife. Alternative blades can be fitted (not supplied). An optional micrometer advanced fracturing tool (12145) is available (in addition to the standard side‐mounted tool). Fractured fragments are captured in the large cold trap located below the specimen stage. Automatic sublimation and sputtering Sublimation temperatures and times can be preset and stored for easy retrieval. The process is fully automatic and graphically displayed on the control screen, showing the actual versus the predicted temperature curve. The high resolution sputter coater is an in‐house design, being based on the market leading Quorum "Q" series of bench top coaters and will give fine grain films essential for FE‐SEM applications. A platinum target is fitted as standard ‐ optional metals include gold, gold/palladium, chromium and iridium. An optional fully integrated carbon fibre evaporation head can also be fitted. An optional terminating film thickness monitor (FTM) is available. The system is fully integrated ‐ no external control boxes. High vacuum performance The preparation chamber is pumped by a remotely‐positioned 70L/s turbomolecular pumping system. Typical preparation chamber vacuums during operation are in the region of 10‐6mbar or better. Positioning the turbomolecular pump away from the SEM ensures total elimination of mechanical vibration and significantly reduces the mass connected to the SEM. A vacuum buffer tank (remotely located in the Prepdek™) is automatically pumped when required. The pumping system is connected to the preparation chamber by stainless‐steel bellows, which allows flexible positioning of the pumping system. A 5m3/hr1 rotary vacuum pump is required to "back" the turbomolecular pump and for slushing and rough pumping operations. The rotary pump can be located up to five metres from the system, allowing remote location if required. Dry pumping alternatives are available. Prepdek™ workstation The Prepdek™ workstation has been designed to allow specimen mounting, freezing (and pre‐frozen specimen manipulation) and transfer device storage on one ergonomically designed work surface. The control electronics are mounted in a sealed, but accessible, cabinet beneath the Prepdek™. A flexible LED light gives the user an excellent view of the preparation process. Conveniently set into the Prepdek™ works surface is a pumped storage tube and allows the cryo transfer device to be stored under clean, dry vacuum conditions when not in use. A variable position specimen shuttle mounting pillar gives a solid base for specimen mounting and includes a height gauge to ensure specimens are within the acceptable height range. The control PC is mounted on a flexible arm and can be positioned to suit the user (eg angled towards the SEM operator during specimen observation ‐ allowing key system parameters to be viewed at a glance). Panel PC touch screen user interface The PP3010T is controlled using a large (15") touch screen panel PC, mounted on the Prepdek™ workstation. User‐defined 'recipes' can be entered and stored for instant future access. The screen can be set to suit operator preferences, for example, vacuum measurement can be displayed in millibar or Pascal. CCD images of the aQuilo cryo preparation chamber and SEM cold stages are displayed and can be expanded to full screen. Although many of the key steps in the specimen preparation process are automated (system set up, sublimation, sputter coating, etc), further help is available through a series of on‐screen videos. SEM cold stage, cold trap and cooling system A highly stable, thermally isolated, nitrogen gas cooled stage attaches to the SEM stage. The SEM stage and cold trap are cooled by two separate cold gas circuits ‐ both capable of reaching temperatures down to ‐190°C or lower. This configuration allows the operator to select stage and cold trap temperatures that are optimised for specific specimens. For example, for some non‐biological materials it is useful to hold the specimen at very low temperatures ‐ for example, a cold stage temperature of ‐175°C. This is possible with the PP3010T as cold trap temperatures of ‐190°C or lower can be selected. The SEM cold stage has a temperature range of 100°C.to ‐190°C and a temperature stability of < 1°C. Compatibility with SEM stage bias mode The PP3010T cold stage is fully compatible with SEM stage bias mode up to 5kV Technical note: gas cooling v conduction cooling Gas cooling allows specimen stage and cold trap (anticontaminator) temperatures down to ‐190°C. In practice, to ensure efficient cold trapping, there needs to be a minimum of 15‐20°C temperature difference between the two. For many standard applications SEM stage temperatures in the region of ‐120°C to ‐140°C are used, with the cold trap set to between ‐170°C and ‐180°C. However, cryo preparation systems are increasingly being use to process and observe frozen vitreous material (eg high pressure frozen specimens) where it is essential to work at temperatures in the range of ‐150°C to ‐165°C in order to prevent the devitrification of ice to its less desirable crystalline state. For such specimens the cold stage can be set to ‐165°C.and the cold trap to ‐190°C. This is not possible with less thermally efficient conduction cooled cryo systems. CHE3010 off‐column cooling The CHE3010 is a fully integrated, remotely mounted cooling system which comes as standard with every PP3010T. The CHE3010T is used to cool the SEM stage, SEM cold trap and aQuilo cryo preparation chamber cold stage and cold traps and will typically reach temperatures down to ‐190°C or lower. The CHE3010 is remotely positioned (typically on the floor behind the microscope) and will run for up to 24 hours between fills. This greatly simplifies the cryo process (no more checking on dewar status and topping up), but also allows overnight, unattended operation ‐ particularly useful for some automated FIB/SEM "slice and view" protocols. Single port interface to the SEM Where SEM geometry allows, both the aQuilo cryo preparation chamber and the SEM cooling system can be fitted to a single chamber port (minimum diameter is 38mm). This gives a compact, tidy installation and frees up a potentially valuable chamber port. Options and accessories Specimen shuttles and stubs The PP3010T is supplied with a selection of holders (please see Specification for details). A range of additional specimen shuttles and stubs is also available. Carbon evaporation and film thickness monitor A carbon evaporation attachment (11920) and a terminating film thickness monitor (12147) can be fitted. Pressurised LN2 dewar The PP7450/75L is a highly recommended option that generates dry nitrogen gas used for cooling the SEM cold stage, cold trap and aQuilo cryo preparation chamber and cold traps. In addition LN2 can be decanted for slushing (freezing). If the PP7450/75L is not included then appropriate, nitrogen cylinder gas can be used. For full details of these and other accessories please see Ordering Information below Installation and training Installation and training typically takes four days and is carried out at the customer site. In most cases installation and training is by specialist Quorum Technologies cryo installation engineers, normally working in conjunction with our local distributor or microscope manufacturer's engineers. Additional Information DownloadsPP3010T one‐year service contact (PDF) PP3010T specimen shuttles and stubs (PDF)Ordering Information PP3010T Cryo‐SEM preparation system for SEM, FE‐SEM and FIB/SEM applications. Including: aQuilo column‐mounted cryo‐preparation chamber with off‐column turbo pumping system. SEM cold stage and cold trap, PrepdekTM workstation with freezing and specimen manipulation facilities, automatic sputtering and sublimation. CHE3010 off‐column gas cooling system. Touch screen, panel PC (15") user interface mounted on the PrepdekTMworkstation. Cryo transfer device, 2 x AL200077B and 1 x 10246 specimen shuttles and the following specimen stubs: E7402 (pkt. 10), 5x E7449, 5x 11541, E7406 (pkt. 5), E7407 (pkt. 5) and 328116510 brass fracturing rivets (pkt. 100). Microscope interfaces, start‐up kit, mounting media and operation manual Pumping The PP3010T requires one 5m3/hr1 rotary vacuum pump (dry pumps available on request) 13034 95m3/hr1 rotary vacuum pump with oil mist filter Options and accessories Highly recommended option: PP7450/75L Pressurised LN2 dewar (75L). Generates dry nitrogen gas used for cooling the SEM cold stage & cold trap and aQuilo cryo preparation chamber and cold traps. Liquid nitrogen can also be decanted for slushing (freezing) Other options and accessories: 11920 Carbon fibre evaporation head and power supply, including 1m high purity carbon fibre (A0819) 12147 Film thickness monitor (FTM). This is fully integrated (no additional boxes) and terminates sputtering at a pre‐set thickness defined by the user 12145 Micrometer controlled fracturing device with tool steel blade. Note: the standard ball‐joint mounted fracturing tool is fitted as standard. The 12145 can be fitted in addition 13060 Two‐years spares/consumables kit Specimen shuttles AL200077B Standard specimen shuttle with insert for 10mm stubs 12434 Specimen shuttle without 10mm hole (flat surface 22mm x 13mm). For large or multiple specimens 10245 Top loading shuttle for "Balzers" style freeze‐facture planchettes or similar small, flat holders or specimens 10246 Top loading 10mm specimen stub shuttle (similar to AL200077B but stub clamping mechanism is located on the top ‐ useful for handling pre‐frozen specimens mounted on a stub) E7433 Rivet holder specimen stub, screw‐down style (for use with 10246) Specimen stubs (all 10mm diameter) E7449 Universal specimen stub with holes and slots 10mm dia. x 7mm high 11541 Universal specimen stub with holes and slots. 10mm dia. x 5mm high E7402 Blank aluminium (Al) stubs (pack of 10) E7403 Blank copper (Cu) stubs (pack of 10) E7405 Screw down stub for thin, hard specimens E7406 Copper (Cu) stubs with 3 x 3mm slots (pack of 5) E7407 Copper (Cu) stubs with 1 x 3mm slot (pack of 5) 32816510 Brass rivets for fracturing liquids (pack of 100) Sputter targets and carbon fibre (all targets 24mm diameter) E7400‐314A Gold (Au) target 0.2mm thick E7400‐314B Gold/palladium (Au/Pd) target 0.2mm thick E7400‐314C Platinum (Pt) target 0.2mm thick E7400‐314F Chromium (Cr) target 0.3mm thick E7400‐314IR Iridium (Ir) target 0.3mm thick A0819 Carbon fibre cord, high purity, for use with optional 11920 carbon attachment (100cm) A0819‐5 Carbon fibre cord, high purity, for use with optional 11920 carbon attachment (5m) NB: For a full quotation, including on‐site installation and customer training, please Contact Us to get in touch with a member of our Sales team, or one of our global distributors.
EMS 5000 Oscillating Tissue Slicer
EC Declaration of Conformity We: Electron Microscopy Sciences Of: P.O. Box 550 Hatfield Pa 19440 Declare that: Equipment: Oscillating Tissue Slicer Model: EMS 5000 Serial Number: ___________ In accordance with the following Directives: 73/23/EEC Low Voltage Directive and its amending directives 89/336/EEC Electromagnetic Compatibility Directive and its amending directives 98/37/EC Machinery Directive and its amending directives Has been designed and manufactured to the following specifications: EN61010-2001: Safety Requirements for electrical equipment for measurement, control, and laboratory use: Part 1 - General Requirements EN50081-1:1992 Electromagnetic Compatibility – Generic emission standard: Part 1 - Residential, commercial, and light industry EN292-1: 1991 Safety of machinery – Basic concepts, general principles for design Part 1: Basic terminology, methodology EN292-2/A1:1995 Safety of machinery – Basic concepts, general principles for design Part 2: Technical principles and specifications I hereby declare that the equipment named above has been designed to comply with the relevant sections of the above referenced specifications. The unit complies with all essential requirements of the Directives. Signed by: __________________________________Date: ____________________ Name: Stacie Kirsch Position: President Done at: 1560 Industry Road Hatfiled, Pa 19440 Phone: 215-412-8400, Fax: 215-412-8450 E-mail:sgkcck@aol.com Website: http://www.emsdiasum.com 0.2 CONDITIONS FOR USE Intended Use The EMS 5000 is intended for acquiring samples for: electrophysiology of fresh tissue, immunocytochemistry and immunohistochemistry, histology, cytology, botanical research, and polymer science. Warnings The EMS 5000 should not be disassembled beyond the indications of this manual. Any disassembly beyond this may affect function and calibration. Do not use phenols or ketones to clean the tray, as it will crack. Care should be taken when handling any blade. Do not contact the front edge of the blade. The EMS5000 should not be placed in the vicinity of high sources of electromagnetic phenomena. If repair is required, please contact EMS at (215) 412-8400 for evaluation and to secure a return authorisation number if necessary. Storage Precautions Store at normal room temperatures between -34°C and 57°C. Do not expose to temperatures below -39°C or greater than 70°C, or a relative humidity of less than 10% or more than 100%, including condensation, or an atmospheric pressure less than 500hPa or greater than 1060hPa for long-term storage. Sterilisation Except for the pedestal and mounting blocks, the EMS 5000 components are not designed for sterilisation. (For questions on sterilisation of the tray components, please contact the EMS Technical Services department at (215-412-8400.) Any attempt to sterilise the non-tray components may result in malfunction or component failure. Handling While a high degree of durability has been designed into the EMS 5000, care should be taken not to drop the components. Do not force the tray on the platform, or the pedestal/vise in the tray. Place all cables and leads where they will not be inadvertently pulled or tangled. 1 OPERATIONAL MANUAL 1.1 FEATURES New slicer head design minimises vibration that causes tissue distortion. User defined "travel window" reduces blade travel time between sections. User selectable multiple slice mode for "hands-free" preparation of many slices. Consistent live tissue slices of 10 microns achieved. Membrane switch keypad, separate from module, isolates circuitry from solutions, reduces “handedness”, and improves ergonomics. Slice thickness up to 999 microns, set in 1 micron increments. Focused LED's mounted to blade arm for constant, direct, cold-source illumination. Two specimen mounting options: XY adjustable pedestal or vise/block arrangement. Removable, easy to clean tray. Accepts Razor, Glass, Diamond, and Sapphire blades. Precise bar graph displays of blade oscillation and advance speeds. Large LED display of slice counter/slice thickness. Removable X2 magnifier lens swings away when not in use. Removable slicer head for cleaning and maintenance. 1.2 DESCRIPTION The EMS 5000 is used for sectioning slice samples for use in the fields of electrophysiology of fresh tissue, immunocytochemistry and immunohistochemistry, histology, cytology, botanical research, and polymer science. The new design of the slicer head minimises Z-axis vibration, which dramatically improves slice surface integrity. This improvement is especially useful for fresh tissue where IR/DIC imaging methods are used. To reduce the blade travel time between slices, the EMS 5000 features a user-programmable travel window. This allows the user to set the range of blade travel customised to the specimen sample, reducing the time between slices. Once the range is set, the START/STOP button is used to toggle the sectioning sequence. The EMS 5000 allows the user to section in two different modes. In manual slice mode, the user can perform all movements of the blade arm using the keypad, or a combination of the keypad and footswitch. In multiple slice mode, the EMS 5000 will automatically perform repeatable sequences. The tray is made of moulded clear polycarbonate for improved viewing of the slice operation. The tray is removable to facilitate specimen mounting and cleaning. Two focused LED’s are installed in the blade head, which provide constant cold illumination of the specimen during the slicing process. Two specimen mounting options are available to the user; a pedestal with forward/backward and side to side tilting adjustments, and a vice and mounting block (2 sizes provided) assembly. The blocks are used to quickly interchange multiple specimens on individual blocks. The front panel of the EMS 5000 has precise bar graph displays of the blade oscillation and advance speeds. A large, three-digit display shows the number of slices performed, and the current slice thickness setting. (The display toggles when either of the thickness keypad buttons are pressed once.) The modular configuration of the keypad allows for ambidextrous and ergonomic operation. The keypad incorporates membrane switch technology for durability and reliability in the laboratory environment. The blade head is removable for occasional cleaning and maintenance. The blade clamp is designed to accept razor, sapphire, diamond, and glass blades. A separate self-contained refrigeration system will be made available soon as an option. 1.3 OPERATING ENVIRONMENT The EMS 5000 has been designed to operate in a typical laboratory setup. It should be placed on a solid, flat surface that is level and free from contaminants and vibration. The EMS 5000 should not be placed or used near or during high sources of electromagnetic phenomena. 1.4 INVENTORY 1.4.1 ITEMS DESCRIBED IN THIS MANUAL 1 ea. EMS 5000 Includes: EMS 5000 Footswitch Tray Magnifier Pedestal Specimen Vise Mounting Blocks (2 sizes) Stainless Steel Slicer Blades (25 ea.) 1/16” Hex Driver Manual (Not Shown) 1.4.2 ADDITIONAL ITEMS REQUIRED FOR OPERATION The following additional items are ORDERED SEPARATELY: 1 ea. 55-XXX Line Cord (Country specific see sec 2.1.4 of this manual for catalog number) 1.4.3 REPLACEMENT ITEMS Stainless Steel Slicer Blades (25ea.) EMS Mounting Blocks (1/2”) EMS Mounting Blocks (1”) EMS Specimen Vise EMS Tray EMS Pedestal EMS Magnifier EMS Footswitch 1.4.4 OPTIONAL ACCESSORIES The following accessories are available: Glass Blade Holder EMS Pedestal Surface EMS Sapphire Blade 1.5 CONCEPTS 1.5.1 DESIGN DESCRIPTION The EMS 5000 slices samples of fresh and fixed tissue, as well as plant and polymer material using an oscillating blade. An embedded microcontroller is used to digitally control functions such as blade position, advance speed, and automatic slice performance. Membrane switch technology in the manual control keypad and polymer packaging protects against corrosion from the laboratory environment. The blade arm is designed to eliminate unnecessary vibration to ensure slice integrity. Cold source focused lighting and a clear tray allow improved visibility. 1.6 TECHNICAL SUMMARY 1.6.1 SPECIFICATIONS EMS 5000: Vertical Travel: 28mm Slice Thickness: adjustable from 1 to 999 microns in 1 micron increments Blade Speed: adjustable from 50 - 5000 cycles per minute Blade Angle Adjustment: adjustable from 15 to 35 degrees Blade Travel: 35mm total Blade Types: Sapphire, Razor, Diamond, Glass Blade Advance Speed: continuously adjustable from 0 – 2.5mm per second Blade Reverse Speed: 2.5mm per second Specimen Size: 25 x 25 x 15mm (W x D x H) maximum volume. Magnifier: 4" diameter, X2 lens mounted on 12" (30cm) gooseneck, 9" working distance Lighting: Focused LED's mounted on the blade arm. Dimensions: Slicer: 27cm x 40cm x 21.5cm Keypad: 17cm x 8.5cm x 3.4cm Weight: 13kg Working space requirements: 12"w x 18"d x 10"h (30 x 45 x 25cm). Power requirements: 85-265VAC, 50-60 Hz, 500mA max universal power input. See Section 2.1.4 for instructions on selecting line cord. Display: 3 segment LED display of slice counter and thickness. (Changes with appropriate buttons.) 20 segment bar graph LED display of blade oscillation and advance speed. Specimen Mount: X, Y axis adjustable pedestal: 1 3/4” x 2 1/2” Mounting blocks: 1/2” x 1/2” x 1/2” (3 ea.) 1” x 1” x 1” (3 ea.) 1.6.2 CONTROLS/CONNECTORS Display – EMS 5000 Front Panel Slice Counter / Thickness: Displays a count of the number of slices from a user defined zero reference. When prompted, displays the slice thickness setting in microns. Oscillation: Displays the speed of the blade oscillation in bar graph form. Each line of the graph represents a division between 0 and 5000 cycles per minute. A blinking bar indicates that the setting is half-way to the next bar. Advance: Displays the speed of the blade advance in bar graph form. Each line of the graph represents a division between 0 and 2.5 mm per second. A blinking bar indicates that the setting is half-way to the next bar. Controls – EMS 5000 Keypad Blade Position : Forward: Moves the blade arm forward at the set advance speed, and begins blade oscillation. Press once to begin movement, again to stop. Reverse: Moves the blade arm back at the maximum travel speed. Press once to begin movement, again to stop. Up: Moves the blade arm up. Momentarily pressing the button moves the blade arm the set thickness amount. Pressing the button for longer than 1 second moves the blade at the fastest travel speed. Down: Moves the blade arm down. Momentarily pressing the button moves the blade arm the set thickness amount. Pressing the button for longer than 1 second moves the blade at the fastest travel speed. Speed: Oscillation: Pressing these buttons increments (+) and decrements (-) the blade oscillation speed. Advance: Pressing these buttons increments (+) and decrements (-) the blade arm advance speed Thickness: Thickness: Pressing these buttons adjusts the slice thickness. Pressing momentarily changes the setting in 1 micron increments. Holding for longer than 1 second causes 10 micron changes. Travel Window: Reverse Limit: Pressing this button sets the position for the blade arm to start the slice process from during multiple slice mode. The limit can be changed by first pressing Start/Stop, then using the blade position buttons to reposition the new limit. Pressing this button again will set the new limit. Forward Limit: Pressing this button sets the position for the blade arm to stop, return to the reverse limit, and start a new slice during multiple slice mode. The limit can be changed by first pressing Start/Stop, then using the blade position buttons to reposition the new limit. Pressing this button again will set the new limit. WARNING: If the Forward Limit is set behind the Reverse limit, the arm will continuously step down without moving forward. If this occurs, press the Start/Stop button to stop movement and correctly set the limits. Multiple Slice: Start/Stop: Pressing this button enable the travel windows, and causes the EMS 5000 to begin slicing in multiple slice mode. Pressing this button once will cause the blade arm to move forward, and oscillate. It will continue until it reaches a forward limit, (travel window or mechanical) stop, return to the reverse limit, step down the selected thickness, and begin another slice. Pressing the button during this process will cause the arm and oscillation to stop and wait for a command. The multiple slice process will continue until the lowest mechanical limit is reached. It will then automatically return to the home position. (full up and back) Warning: Due to the variance in blade angle settings, it is possible for the blade to run into the pedestal or mounting block surface before reaching the lowest mechanical limit. Zero: Pressing this button resets the slice counter display to read all "0". Home: Pressing this button will cause the blade to return to its full up and full back "home" position. Blade arm needs to be at rest before pressing the Home button. 1.6.3 COMPATIBILITIES The EMS 5000 is compatible with most commercially available glass, razor, sapphire, and diamond blades. For questions on blade compatibility, contact the EMS at 215-412-8400 1.7 ILLUSTRATIVE PROCEDURE The EMS 5000 can be operated in two modes. In manual slice mode, the user can perform all movements of the blade arm using the keypad, or a combination of the keypad and footswitch. In multiple slice mode, the EMS 5000 will automatically perform repeatable sequences. 1. Ensure that the EMS 5000 is installed correctly. 2. Activate power to the unit and position the blade arm at the full up and back (“Home”) position by pressing the HOME button on the keypad. 3. Remove the tray from the unit by loosening the two thumbscrews on both sides of the bottom of the tray. Slide the tray forward off of the base. 4. Remove the pedestal from the tray. The pedestal can be removed by loosening the post thumbscrew a half turn. Grasp the thumbscrew and pivot pin, lift the pedestal straight up. Alternately, remove the vise in the same manner if using the vise/mounting block arrangement. 5. Prepare the bath medium. Set aside a small amount in a beaker. Place both in an ice bath or other to cool. 6. Clean the pedestal surface or mounting block. Ensure that the surface is completely dry. 7. Rapidly excise the tissue. Cut the structure of interest into a block leaving a minimum of excess (particularly connective) tissue. Continuously bathing the tissue with an eyedropper of cooled medium during the trimming process is suggested. 8. Turn the side of the tissue to be bonded up and blot as dry as possible. Apply a thin layer of tissue adhesive to the pedestal or block surface, and immediately position the dry side of the sample on it, ensuring that a strong bond is made. 9. Position the pedestal in the tray. Orient the pedestal angle if desired. Alternately secure the mounting block in the vise. Position the vise/block setup in the tray. 10. Immediately fill the tray with cooled, oxygenated medium. Ensure that the sample is covered by a millimeter or two. 11. Position a new blade in the blade holder and tighten securely. Cleaning both slides of the blade with acetone or alcohol is suggested. Set the blade angle, being careful not to touch the blade edge. 12. Set the Advance speed to 15-20, using the appropriate keypad buttons, to position the blade to an initial slicing position behind the sample. 13. If slicing in Multiple Slice mode, set the reverse limit at this point by pressing the REVERSE LIMIT button in the TRAVEL WINDOW section of the keypad. 14. Set the Oscillation and Advance speeds using the appropriate keypad buttons to perform the initial slice. It is a good practice to make the first slice rather large to initially prepare the sample. 15. Press the FORWARD button on the keypad once to begin the slice process. When the blade has cleared the slice sample, press the FORWARD button again to stop. 16. If slicing in Multiple Slice mode, set the forward limit by pressing the FORWARD LIMIT button in the TRAVEL WINDOW section of the keypad. 17. Once the initial slice is performed, press the REVERSE button once to return the blade to a convenient position behind the sample. Press the REVERSE button again to stop movement. Alternately, press the START/STOP button to reposition the blade to the travel window Reverse limit. 18. Reset the Oscillation and Advance speeds if desired to begin performing slices of the desired thickness in either Manual or Multiple Slice mode. 19. Perform slices by either manually performing all blade arm movements using the keypad (or footswitch and keypad combination), or using the START/STOP button to begin slicing within the set Travel Window. 20. When slicing and harvesting is completed, discard the medium and any unused samples. Remove the pedestal or vise/block from the tray. Clean the surface of the pedestal or block by scraping with a used blade and wash with alcohol. 2 REFERENCE MANUALS 2.1 REFERENCE INFORMATION 2.1.1 PACKAGING The EMS 5000 is enclosed in a two-piece (bottom and back, top) ABS UL94V0 plastic enclosure. The overall dimensions are 27cm x 40cm x 21.5cm (10.5" x 16" x 7.5") The top portion is connected to the interior chassis on the top of the unit, the back panel, and the bottom portion of the case through the baseplate. 2.1.2 MOUNTING The EMS 5000 is designed as a stand-alone unit. It is not designed to be rack mounted. 2.1.3 INSPECTION EMS Modules are factory checked and calibrated but should be carefully inspected upon receipt, before using, or activating power. If any exterior damage to the shipping carton is noted, the instrument(s) should be inspected for obvious physical damage. The contents of each package should be physically checked against the inventory list (sec. 1.3) to determine shortages or errors in inventory. 2.1.4 POWER CONNECTIONS The EMS 5000 is powered through an internal 12V power supply. (input:85-265VAC, 50-60Hz, 500mA max) An international pattern Line Cord (not shown) is ordered separately, and is specified by country per the catalog number. (See table below for catalog numbers.) The power entry uses double pole switching and fusing. (2 ea. 5X20, 1/2-amp slo-blo fuses) Contact Technical Services at 215-412-8400 for assistance. 55-AUS Australia 55-CH China 55-DAN Denmark 55-EURO Europe 55-ISR Israel 55-ITA Italy 55-JA Japan 55-SAF South Africa 55-SWI Switzerland 55-UK United Kingdom 55-USA North America To replace fuses: 1. Remove line cord if applicable 2. Remove fuse drawer located between the line cord entry and power switch by squeezing the tabs on each end of the drawer, and pulling out. 3. Replace fuses with the correct value (2 ea. 5X20, 1/2-amp slo-blo) 4. Insert drawer back into slot until tabs “click”. 2.1.5 WARRANTY All EMS products are unconditionally guaranteed against defects in workmanship for one year from date of shipment as long as they have been exposed to normal and proper use. Although the one- year warranty may have expired, please contact our Service Department before attempting any repairs or alterations. Many of these repairs will still be performed at the factory at no charge to the customer. 2.1.6 POLICIES 1. TECHNICAL SUPPORT: It is our policy to provide our customers with the most comprehensive technical support in the industry. If any questions arise or problems occur, we encourage you to call or write and we promise to promptly and comprehensively respond to your requirements. 2. TRADE-UP POLICY: It is our policy to offer customers trade-up ability as new and/or expanded capabilities for their instruments are announced. In many cases, full credit will be given. In general, we will allow 100% credit for two years and depreciate 20% per year thereafter. Please contact our Marketing Department for information relating to your particular situation. 2.1.7 SERVICE Should service be required, please contact our Service Department for a return authorization number and instructions (215-646-1566). Please have the serial number on hand (located on the back panel). Carefully pack the instrument before returning. Please include a note indicating: 1. The model number and purchase date of the instrument 2. The person to contact if questions arise 3. A note stating that the unit is free of biological contaminants and the "symptoms" indicating that repair is necessary If the instrument is not covered by the warranty, a quotation will be forwarded to the sender detailing the repairs necessary and charges, before repair is begun. 2.2 INSTALLATION Initial Installation: 1. Set the unit on a solid level surface. 2. Install the line cord into the receptacle on the back of the unit. 3. Ensure power switch on the back panel is in the "Off" position. (Indicated by the O side of the rocker switch pressed in.) 4. Plug the line cord into a into a properly grounded wall receptacle. Foot Pedal Installation: 1. Ensure power switch on the back panel is in the "Off" position. (Indicated by the O side of the rocker switch pressed in.) 2. Install the foot switch plug into the ¼” jack located on the back panel. 2.3 FUNCTIONAL CHECKOUT 1. Ensure that the unit is installed correctly per section 2.2. 2. Activate power using switch on the back panel. (Indicated by the l side of the rocker switch pressed in.) 3. Ensure that the LEDs in the front of the blade head are illuminated. 4. Ensure that the three-digit display illuminates, and reads “000”. If the display reads otherwise, press the ZERO button and ensure that it displays “000”. 5. Ensure that both bar graph LED’s are at 10 if this is the initial checkout. If this is a yearly checkup, use the appropriate SPEED buttons to set both displays to 10. 6. Press the OSCILLATION “+” button repeatedly to advance the display to 15. Ensure that the display moves up and alternates between blinking and moving to the next bar. Hold the “+” key down. Ensure that after one second the display advances rapidly to the top (20). 7. Press the OSCILLATION “-” button repeatedly to retract the display to 15. Ensure that the display has moved down and alternates between blinking and moving to the next bar. Hold the “-” key down. Ensure that after one second the display retracts rapidly to the bottom (0). 8. Repeat steps 6 and 7 using the ADVANCE “+” and “-“ buttons. When complete use the appropriate buttons to return both displays to 10. 9. Press either of the THICKNESS buttons once. Ensure that the three-digit display reads “100” if this is the initial checkout. If this is a yearly checkup, use the appropriate THICKNESS buttons to set to 100. 10. Press the THICKNESS “+” button repeatedly. Ensure that the display increments by one with each press of the button. The first press would cause the display to show the current thickness if it was previously showing the slice count. Hold the THICKNESS “+” button down. Ensure that after one second the display advances rapidly. 11. Press the THICKNESS “-” button repeatedly. Ensure that the display decrements by one with each press of the button. The first press would cause the display to show the current thickness if it was previously showing the slice count. Hold the THICKNESS “-” button down. Ensure that after one second the display advances rapidly. 12. Using the THICKNESS buttons, set the slice thickness to 500. 13. Press the HOME button. If this is the initial checkout, no movement of the blade arm should occur. If this is a yearly checkout, the blade arm may return to its home position if it was not previously returned to it. 14. Press the DOWN BLADE POSITION button once. You should observe the blade arm moving down. Ensure that the Slice Counter display has incremented by one. Hold the DOWN button. Ensure that the blade arm moves continuously down. Releasing the DOWN button stops movement. 15. Repeat #14 with the UP button. Ensure that the blade arm moves up a step amount when pressed once, and moves up continuously when held down. 16. Press the FORWARD button once. Ensure that the blade begins oscillating and moving forward. Press the FORWARD button again, ensure that the blade stops all movement. 17. Press the HOME button once. Ensure that the blade arm moves to its full up and back position. 18. Press the START/STOP button. Ensure that the blade arm steps down, begins oscillating and moving forward, and that the slice counter increments by 1. 19. Allow the blade arm to travel to its forward mechanical limit. At that point the blade should stop oscillating and return to the reverse limit. Ensure that at the reverse limit, the blade arm stops, steps down, then begins oscillating and moving forward. Ensure that the slice counter increments by 1. 20. While the arm is still moving, press the START/STOP button. Ensure that all movement has stopped. 21. Press the START/STOP button again. Ensure that the blade arm returns to its reverse limit, stops, steps down, and begins oscillating and moving forward. 22. Press the START/STOP button to stop movement when the blade is approximately ¼ of its travel. 23. Press the REVERSE LIMIT button to set the rear slice window limit. 24. Press the FORWARD button and allow the blade to travel to approximately ¾ of its travel. Press the FORWARD button again to stop movement. 25. Press the FORWARD LIMIT button to set the forward slice window limit. 26. Press the START/STOP button. Ensure that the blade travels back to the set rear slice window limit that was set, step down (counter increments by one), and begin oscillating and moving forward. When it reaches the set forward slice window limit, ensure that the unit stops and returns to the reverse limit where it cycles again. 27. Press the START/STOP button to stop the cycle. Using the FORWARD and REVERSE buttons to position, set a new travel window using the FORWARD LIMIT and REVERSE LIMIT buttons. 28. Press the START/STOP button and ensure that the unit cycles in the new set window. Press again to stop the cycle. 29. Press the HOME button to return the unit to its full up and back position. Slice Test: Below is a test to determine integrity of the blade arm movement. It can also be used to determine the correct oscillation and advance speed settings without using specimen tissue. 1. Cut a block of hard-boiled egg white approx. 1.5cmX1.5cmX1.5cm 2. Glue the block to the center of the platform surface (cyanoacrylate works fine in this application). Alternately, attach the egg to a mounting block if using the vise/block arrangement. 3. Install the platform or vise/block into the tray. Fill the tray with cold water. 4. Install the tray onto the unit. 5. Install a new blade into the blade holder set the angle to 25°. 6. Position the blade to a point behind the egg block. Press the REVERSE LIMIT button to set the rear slice window limit. 7. Position the blade using the DOWN button to a point just above the egg block. 8. Press the FORWARD button and allow the blade to travel over the egg block. 9. When the blade has cleared the block, press the FORWARD button again to stop movement. 10. Press the FORWARD LIMIT button to set the forward slice window limit. 11. Set the thickness to 100 microns. 12. Set the oscillation and advance speeds to a low number (10 is a good starting point) 13. Press the START/STOP button and allow the unit to cycle through until it makes uniform slices of the egg block. Press START/STOP button to stop movement. 14. Set the thickness to 30 microns. 15. Cycle the unit until uniform slices are achieved. The oscillation and advance speeds may need to be changed if the slice integrity is not good. 16. Once the 30 micron slices are achieved, set the thickness to 20 microns and repeat until the slices are uniform. Again, the correct oscillation and advance settings need to be determined empirically. A general rule is that the oscillation and advance need to be slow if thin (40micron or less) slices are desired. 2.4 OPERATIONAL INFORMATION The EMS 5000 can be operated in two modes. In manual slice mode, the operator may make a slice and harvest it while the blade arm repositions itself for the next slice. In multiple slice mode, the operator may program the EMS 5000 to automatically slice the specimen block into multiple uniform slices. Single slices can also be performed in multiple slice mode by using the start/stop button. Initial Setup: 1. Ensure that the unit is installed correctly per section 2.2 2. Activate power using switch on the back panel. (Indicated by the l side of the rocker switch pressed in.) 3. Press the HOME button to ensure that the blade arm is in the maximum up and back position. If it is already in the home position, no movement will occur. 4. Remove the tray from the unit. To remove the tray; loosen the two thumbscrews on both sides of the bottom of the tray. Slide the tray forward off of the base. 5. Remove the pedestal from the tray. The pedestal can be removed by loosening the post thumbscrew a half turn. Grasp the thumbscrew and pivot pin and lift the pedestal straight up. Alternately, remove the vise in the same manner if using the vise/mounting block arrangement. 6. Prepare a volume of Ringers or other medium oxygenated with 95% O. Put a small amount in a beaker immersed in an ice bath. 7. Secure a new blade in the blade holder and tighten securely. Cleaning the blade with acetone or alcohol is suggested to remove any grease or oil. 8. To set the blade angle; loosen the black Allen screw on the blade arm. Reference marks on the side of the blade head are provided at 15,25, and 35 degrees. Once the angle is set, re-tighten the Allen screw to secure. The correct blade angle will vary depending on blade dimension, sample type, and desired slice thickness. The angle should be set so that the slice “peels” up and over during the slice process. 9. Prepare the surface of the sample pedestal or mounting block by scraping with a previously used slicer blade followed by a light sanding with fine emery paper; clean with alcohol and blot absolutely dry. 10. Rapidly excise the tissue and cut the structure of interest into a block leaving a minimum of excess tissue, especially connective tissue. During the trimming process, bathe the tissue continuously with an eyedropper filled with the cooled medium. 11. Turn the side of the tissue to be bonded up, and gently blot as dry as possible. Place a thin layer of tissue adhesive on the pedestal; too thick a layer of adhesive will ride up along the sides of the tissue and interfere with the slicing. Immediately position the dry side of the sample on the adhesive and ensure a solid bond is created. 12. Position the pedestal in the tray and set the sample angle if desired. To set the sample angle; use the pivot pins on the side (y axis) and front (x axis) to set the angle, then secure the pedestal by tightening the thumbscrew. Alternately, secure the mounting block in the vise, and position the setup in the tray. 13. Immediately fill the tray with cooled medium. Ensure that the sample is covered by one or two millimeters. 14. Slide the tray onto the mount and tighten the two thumbscrews to secure. 15. Set the ADVANCE speed to a high setting (15-20) using the appropriate keypad buttons. 16. Using the BLADE POSITION buttons, position the blade to an initial slicing position just behind and above the sample. 17. Set the OSCILLATION and ADVANCE speed using the appropriate keypad buttons. 18. Set the slice thickness for the initial slice using the appropriate keypad buttons. In order to initially prepare the sample, it is suggested that the first slices are relatively thick (50 microns and up). 19. Press the FORWARD button once to initiate slicing, Once the blade has cleared the sample, press the FORWARD button again to stop. 20. Press the REVERSE button to reposition the blade behind the slice. When the blade is in a convenient position, press the REVERSE button again to stop movement. 21. Press the DOWN button once to move the blade arm down at the chosen thickness setting. 22. It may be necessary to perform multiple slices to initially prepare the sample. Once a consistent slice is achieved, usable sample slices can be performed by one of the following slice modes. Manual Slice Mode: 1. Position the blade at a convenient spot behind the slice. 1. Press the ZERO button to set the slice counter to zero if desired. 2. Set the slice THICKNESS using the appropriate keypad buttons. 3. Set the OSCILLATION and ADVANCE speeds to an appropriate setting. Due to differences in blade and sample type, these settings will be obtained empirically. Generally, the thinner the slice, the slower the speeds. 4. Press the FORWARD button once to begin slicing. 5. Once the blade has cleared the sample, press the FORWARD button once to stop movement. If allowed to continue, the blade will advance to its forward mechanical limit then stop and wait for a command. 6. Press the REVERSE button once to move the blade back. Harvesting the slice can be performed if desired. 7. Once the blade has cleared the slice, press the REVERSE button once to stop movement. If allowed to continue, the blade will return to its reverse mechanical limit, then stop and wait for a command. 8. Press the DOWN button once to lower the blade arm the set thickness amount. 9. Repeat steps 4 – 8 to repeat slicing. 10. To return the blade arm to its furthest up and back position, press the appropriate BLADE POSITION button to stop movement if in motion (FORWARD if blade arm is advancing, REVERSE if returning) then press the HOME button. 11. To check or change the slice thickness setting at any time, press either of the THICKNESS buttons once. The display will show the current slice thickness in microns. Pressing either again will change the slice thickness. The new setting will take effect the next time the DOWN button is pressed. 5 seconds of inactivity will cause the display to revert to the slice count mode. 12. The foot pedal may be used in manual slice mode to take the place of the FORWARD blade position button on the keypad. (These are disabled while the pedal is installed. See sec. 2.2 for instructions on installing the foot pedal.) Pressing the foot pedal causes the blade to oscillate and move forward. Letting up will cause the blade to return to the reverse limit and stop. (If blade reaches its forward mechanical limit, it will automatically return.) Multiple Slice Mode: To set the TRAVEL WINDOW: (Optional. The travel window can be used to reduce the operating range to save time. If the travel window is not used, the forward and reverse mechanical limits define the travel window.) 1. Position the blade at a convenient spot behind the sample. 2. Press the REVERSE LIMIT button if not previously set during initial setup. During operation the blade will return to this point when slice is complete. 3. Press the FORWARD button once to begin slicing. 4. Once the blade has cleared the sample, press the FORWARD button once to stop movement. If allowed to continue, the blade will advance to its forward mechanical limit, then return to the reverse limit and wait for a command. 5. Press the FORWARD LIMIT button if not previously set during initial setup. During operation, the blade will automatically return to the reverse limit from this point. 6. To change the Forward or Reverse limits at any time during operation, press the START/STOP button once to stop movement. Reposition the blade arm using the appropriate keypad buttons. Repeat steps 2-5 to set the new slice window. 7. Once both limits are set, the unit is ready to automatically slice multiple slices. To begin slicing: 1. Press the ZERO button to set the slice counter to zero if desired. 2. Press the START/STOP button once. The blade will oscillate and move forward until it reaches the set forward limit (or mechanical), stop, then return to the reverse limit. 3. When the reverse limit is reached, the blade arm will stop, step down the set thickness amount, and move forward for another slice. 4. The unit will continue automatically slicing, until the lowest mechanical limit is reached, or the START/STOP button is pressed. Due to the variance in blade angle settings, it is possible for the blade to run into the pedestal or mounting block surface before reaching the lowest mechanical limit. 5. The START/STOP button can be used to perform single slices. Pressing the START/STOP button once will stop movement of the blade arm if in motion. If not in motion, pressing the START/STOP button will cause the blade arm to move to the reverse limit, either mechanical or set, step down the set thickness amount, then begin slicing. 6. To check the slice thickness at any time, press either of the THICKNESS buttons once. The display will show the current slice thickness setting in microns. 5 seconds of inactivity will cause the display to revert to the slice count mode. 7. To change the thickness setting, press the START/STOP button once to stop movement if in motion. Change the setting using the appropriate keypad buttons, then press the START/STOP button again. The blade arm will return to its reverse limit, step down the new thickness amount, and begin slicing. 8. Pressing the HOME button at any time will return the blade arm to its furthest up and back position. Foot Pedal Operation 1. Turn the power switch off if applicable 2. Ensure that the foot pedal is installed correctly per section 2.2. 3. Activate power using switch on the back panel. (Indicated by the l side of the rocker switch pressed in.) 4. Press the START/STOP button to activate multiple slice mode. (The foot pedal needs to be in this mode to function correctly.) 5. The foot pedal is used in place of the FORWARD keypad button in multiple slice mode above. 6. Pressing the foot pedal down begins blade arm advance and oscillation. Lifting off stops all movement. 2.5 SCHEDULED MAINTENANCE The surfaces of the EMS 5000 can be cleaned of any salt buildup etc. by washing with soap and water. DO NOT use phenol or ketones to clean. A yearly performance of the Functional Checkout in section 2.3 should be performed to ensure function and calibration. If the unit fails any part of this functional test, contact the EMS Repair Department at 215-412-8400
EMS Poly III
Which solvents can be evacuated with the EMS Poly III? The instrument handles any of the solvents currently used in embedding protocols. In principle any solvent can be safely and gradually evaporated as long as the proper settings for pressure vs temperature are observed. The one important rule is that the pressure should initially NEVER be lower than the saturated vapour pressure at that temperature. Values can be calculated with the Antoine Equation or taken from a graph. For the bulk removal of solvent it is advised to work at a pressure that is approximately 10% higher than the saturated vapour pressure. Failing to do so may lead to sudden boiling of the solvent and spilling of mixture and specimens from the vials. The risk of sudden spilling is higher with more volatile solvents such as propylene oxide and instrument settings need to be accurate. Important: at no stage should solutions be allowed to bubble. Gas bubble formation needs to be avoided to prevent splashing and damage to the specimens when bubbles should form inside the specimen. Although extensive testing has been carried out, specific combinations of solvent and resin need to be tested for their suitability for embedding and polymerisation in the EMS Poly III on an individual basis. The EMS Poly III instrument is capable of effectively removing any of the solvents commonly used in embedding as long as the following rules are observed: Use small tissue pieces, smaller volume of cell pellets Use separate programs for embedding and polymerisation Use the smallest amount of resin in the embedding step During polymerisation do NOT lower the pressure to a level equal to or lower than the saturated vapour pressure as any trace amount of sol-vent will result in the formation of holes in the specimen Small specimens are more easily embedded because of their favourable surface/volume ratio. Likewise, solvents are more easily extracted. Along those lines it is obvious that solvent is more easily removed if the specimens are covered (or even just merely contained) in a minimum amount of resin. For instance, when using BEEM capsules it is typically sufficient to end up with 30-50µL of resin at the end of the embedding run. Using a dilute resin/solvent mixture, or a small volume of mixture in the embedding program leaves the specimens covered in a thin layer of resin by the end of the embedding run, just enough to cover or contain the specimen. A procedure that has been tested for embedding yeast cells is described below and may serve as a starting point. Yeast cells were fixed with glutaraldehyde in cacodylate buffer, washed in distilled water and postfixed with 1% KMn04 in distilled water. After washing in distilled water the cells were dehydrated in a graded series of solvent/water mixtures: 50% - 70% - 80% - 90% -100% - 2xl00% absolute. Each step lasted 10min. Cells are then resuspended in resin/solvent 1:10 (v/v) and after 10 min. pelleted. The pellet is resuspended in fresh mix (approx the volume of the pellet) and then transferred into one or more BEEM capsules. It is strongly recommended to transfer the suspension into BEEM vials OUTSIDE the EMS Poly III vacuum recipient to avoid spilling of resin inside the recipient. Any spilled resin on the vials should also be removed before inserting the BEEM vials into the recipient recessions. Once all the BEEM vials have been transferred, the embedding program can start. The embedding program is followed by the polymerisation program, after the BEEM vials have been topped off with pure resin. Alternately, silicone moulds can be used in the polymerisation program.
EMS Tissue Slicers
The EMS Oscillating Tissue Slicers for the most challenging of sectioning with the highest quality. The EMS 4500 and 5000 Oscillating Tissue Slicers represent the most thoughtfully designed solution to sectioning either fixed or fresh tissue, as thin as 5 microns. No longer is there a need for embedding or freezing your sample and preparation time is reduced dramatically. The risks of distortion and artefacts normally associated with these procedures are eliminated. The EMS 4500 and 5000 meet the highest standards for precision and accuracy and meet or exceed the performance of units on the market costing twice as much. The EMS Tissue Slicer Family The EMS 5000 series may be used for the sectioning of samples for use in the fields of electrophysiology of fresh tissue, immunocytochemistry and immunohistochemistry, Neurophysiology, Neuropathology, Experimental Biology, Histology, Cytology, Botanical Research, Microscopy, Polymer and Materials Science. The EMS 5000 now features a new innovative design of the slicer head which minimises Z-Axis vibration. This improvement dramatically improves slice surface integrity and is especially beneficial in the field of fresh tissue where IR/DIC imaging methods are concerned. The unique blade holder has now been modified to accept: razor, sapphire, diamond and glass blades. To reduce time between slices the EMS 5000 series features a user programmable travel window (cutting window). This allows the sectioning cutting range of the blade to be customised to the specimen sample. This feature of "automatic sectioning" offers a beginning advance and end advance further automating the EMS 5000. In conjunction with our "home button" the programmable window guarantees rapid sectioning of even the largest of specimens. As well, the EMS 5000 allows the sectioning of samples in two different modes. In single slice mode, the unit sections samples and harvests them while the blade repositions itself to perform the next slice and in the multiple slice mode the EMS 5000 will automatically perform multiple uniform slices. The EMS 5000 series control panel has been totally redesigned to allow for ambidextrous and ergonomic operation. The keypad has been sealed to avoid buffer spill seepage. The tray is made from moulded clear polycarbonate for improved viewing of the slicing operation. The tray is removable to facilitate specimen mounting and cleaning. Two white LED's are installed in the blade head, which provide constant cold illumination of the specimen during the slicing process. The EMS 5000 series is engineered for maximum stability ensures even the most delicate of tissue to be cut successfully every time. The knife holder allows any blade (as per list) to be secured and the blade angle set from 10- 30 degrees. The specimen mount is x, y, z axis adjustable and the knife advance is continuously adjustable with a touch of the keypad from 0- 5mm/s and the section thickness is now adjustable in 1 micron increments ranging from 1-999 microns. Our precision slicer features automatic touch selection of section thickness, blade speed, advance, and a user-defined travel window so that the consecutive sections may be cut easily and rapidly. Other EMS 5000 features include: Touch pad control of the blade height, slice counter / slice thickness, precise bar graph displays of blade oscillation and advance speeds. Blade oscillation is adjustable from 50-5000 cycles/minute and advance speed is adjustable continuously from 100 microns -5mm/sec. Our EMS 5000 series is designed for easy operation and maintenance. The specimen collecting tray is removable and easy to clean. All controls may be set with one hand with options for multiple slicing modes. The unit comes complete with gooseneck magnifying lens, media tray (2.5" wide) and pedestal, specimen vice holder, blocks, as well as a foot switch with 6' cable. A large tray (3.5" wide) is available as an option and comes complete with fixed stage and adjustable pedestals. The tissue pedestal with pivotal pin (included with media tray), allows for the mounting of a specimen directly to the pedestal and the ability to change the angle of your specimen relative to the blade, offering you perfect specimen orientation. The sample vice with pivotal pins (also included in package) allows for the adherence of your specimen directly to a mounting block. The EMS 5000 series meets the highest standards for precision and accuracy. It meets or exceeds the performance of units on the market costing twice as much. Updates and Advantages of the EMS 5000 Series Cutting window for automatic sectioning allows for faster and more reliable sectioning. Control pad with membrane buttons is now separated from the slicer module improving ergonomics and reducing buffer spillage from contaminating the controls. X,Y, and Z - 3 axis adjustable specimen mount. Easy to release slicer head for safety and ease of specimen mounting. Slice thickness is now adjustable from 1-999 in 1 micron steps. One touch "home" button to return the blade arm to a predetermined point near the specimen. The specimen tray is made from a clear polycarbonate for improved viewing of the slice operation. No worry of leakage and section visualisation in easy. The pivot pins which come as a standard on the pedestal as well as the vice holder allow changing of the angle of the pedestal relative to the blade. Easy sample orientation in all directions. The tray includes a lock on feature for a more secure mount to the platform. The standard unit comes complete with our unique pivotal tissue pedestal (you can mount the specimen directly on the pedestal without the need for vices or blocks mounted in the sample tray). The EMS 4500 Tissue Slicer The EMS 4500 Tissue Slicer is a scaled down version of the EMS 5000. All of the specifications of the EMS 5000 are the same for the EMS 4500. The only difference between the two units are as follows: The EMS 4500 does not include: Functional Features Accessories Adjustable window Multi-Slice function Home functionality Foot Switch Tissue Blocks Vice Holder The EMS 4500 can be upgraded to the complete EMS 5000 unit at any time. The EMS 5000 Refrigerated Controller and Tray The EMS 5000 Refrigerated Controller and Tray provide refrigeration capabilities to the 5000 Tissue Slicer. The controller three digit LED displays the current temperature of the bath medium in degrees C. The temperature is maintained within 0.5°C. The temperature range on the unit is between 0-10°C. The refrigerated tray is fitted with a peltier heat exchange unit. A cold water source is circulated through the assembly to transfer heat. The tubing is connected to the tray with quick connect fittings. No tools are necessary for installation. The fittings are self sealing to prevent leaking during installation. The tray fits directly to the mounting platform of the EMS 5000. Refrigeration Controller/Tray Slice Counter/ Thickness Displays a count of the number of slices from a user defined zero reference. When prompted, displays the slice thickness setting in microns. Advance Displays the speed of the blade advance in bar graph form. Each line of the graph represents a speed of xxx. Oscillation Displays the speed of the blade oscillation in bar graph form. Each line of the graph represents a speed of xxx. Features: Control pad with membrane buttons separated from the slicer module to improve ergonomics, reduce "handedness", and isolate switches from bath solutions. New slicer head design increases blade motion stability and minimises vibration that causes tissue distortion. Slice thickness from 0 to 999 microns, set in 1 micron increments. Push button advance; digital display of status. Easy release slicer head facilitates safe, easy specimen mounting as well as easy cleaning. Ultra light blade oscillation mechanism Accepts metal, glass, diamond and sapphire blades 3 axis adjustable specimen mount (X,Y,Z). User defined "travel window" reduces blade travel time between slices. User selectable slicing modes for "hands-free" performance of many slices. Slice counter/Slice thickness display Precise bar graph displays of blade oscillation and advance speeds. Consistent live tissue slices of 10 microns achieved. Adjustable/Removable magnifier lens X2-Swings away when not in use. White LED's mounted to knife holder for constant, direct, cold source illumination during slicing. Blade mechanism above bath simplifies maintenance; easy to clean removable tray. Mounting of the specimen can be done directly on our unique tissue pedestal (with the option of using the pivot pin [see diagram 1] or just setting the pedestal flat on the bottom of the tray on a 90° angle [see diagram 2] in addition a sample vice holder, with pivot pin can be attached to the tray and mounting blocks used [see diagram 3] - both devices come standard with unit). Adjustable knife angle indicator is built into the arm of the unit. Specifications: 5000 Series Vertical travel 28mm Slice Thickness: Adjustable from 1 to 999 microns in 1 micron increments Blade Speed Adjustable from 50- 5000 cycles per minute Blade Angle Adjustment Adjustable from 15 to 35 degrees Blade Travel 35mm total Blade Advance Speed Continuously adjustable from 100 microns-5mm per second Blade Types Sapphire, Razor, Diamond, Glass Blade Reverse Speed 2.5mm per second Specimen Size 25mm Wide x 25mm Diameter x 15mm High maximum volume Magnifier 4" diameter, X2 lens mounted on 12" (30cm) gooseneck, 9" working distance Lighted White LED's mounted on the knife holder aimed at blade edge. Dimensions Slicer: 27cm x 40cm x 21.5cm Keypad: 17cm x 8.5cm x 3.4cm (6.7" x 3.4" x 1.35") Weight ~13kg (30 lbs) Power Requirements 110-240VAC, 50-60Hz universal power input. Available with CE Mark Display 3 segment LED display of slice counter and thickness. (Changes with appropriate buttons) 20 segment LED's bar graph display blade oscillation and advance speed. Specimen Mount X, Y, Z axis adjustable pedestal: 1 3/4" x 2 1/2" Working Space Requirements 12" Wide x 18" Diameter x10" High) (30cm x 45cm x 25cm) Mounting Blocks 1/2 x 1/2 x 1/2 " (3 each) /1 x 1 x 1" (3 each) Specifications: 5000 Series Refrigeration Controller and Tray Temperature Control Range 0° - 10°C Temperature Indication 3 digit LED display indicates temperature in 0.1°C with a negative sign indicator. Power Requirements 115/230V AC 50-60 Hz auto ranging fused at 1 amp max current Dimensions Control Module: 8.25 x 6.25 x 5.5" (20.9cm x 15.9cm x 13.9cm) Cooling Tray: 4.25 x 3.5 x 2.625 " (10.8cm x 8.9cm x 6.7cm) with a 3' cable (91.4cm) Control Keypad Blade Position Forward: Moves the blade arm forward at the set advance speed. Press once to begin movement, again to stop. Reverse: Moves the blade arm back at the maximum travel speed. Press once to begin movement, again to stop. Up: Moves the blade arm up. Momentarily pressing the button moves the blade arm the set thickness amount. Pressing the button for longer then one second moves the blade at the fastest travel speed. Down: Moves the blade arm down. Momentarily pressing the button moves the blade arm the set thickness amount. Pressing the button for longer then one second moves the blade at the fastest travel speed. Speed Oscillation: Pressing these buttons increments ( ) and decrements(-) the blade oscillation speed. Advance: Pressing these buttons increments( ) and decrements (-) the blade arm advance speed. Thickness Pressing these buttons adjusts the slice thickness. Pressing momentary changes the setting in 1 micron increments. Holding for longer then 1 second causes 10 micron changes. Travel Window Reverse Limit: Pressing this button sets the position for the blade arm to start the slice process. The limit can be changed using the blade position buttons at any time to override the limit. Pressing this button again will set the new limit. Forward Limit:Pressing this button sets the position for the blade arm to stop and return to the reverse limit. The limit can be changed using the blade position buttons at any time to override the limit. Pressing this button again will set the new limit. Multiple Slice Start/Stop: Pressing this button starts the process of automatically slicing. Pressing this button once will begin the blade arm moving forward. It will continue until it reaches a forward limit (travel window or mechanical) stop, return to the reverse limit, step down the selected thickness, and begin another slice. Pressing this button during this process will cause the arm to stop and wait for a command. The multiple slice process will continue until the lowest mechanical limit is reached. It will then automatically return to the home position (full up and back). Zero Pressing this button resets the slice counter display to read all "0". Home Pressing this button at any time will cause the blade to return to its full up and full back "home" position. Different Mounting Options Mounting of the specimens can be done directly on our unique tissue pedestal with the option of using the pivot pin (see diagram1) or just setting the pedestal flat on the bottom of the tray on a 90° angle (see diagram2). In addition, a sample vice holder, with pivot pin can be attached to the tray and mounting blocks used (see diagram3). Both devices come standard with the EMS 5000. The cutting angle can be easily changed and calibrated by loosening the set screw on top of the support arm. The angles between 10-20° can be read off the scale on the side of the support arm. Diagram One Standard Pivoting Tissue Pedestal The pivot pedestal arrangement is designed so that the sample can be glued directly to the surface and the angle of the sample can be adjusted relative to the blade. Diagram Two Standard Tissue Pedestal This is the same pedestal as in diagram one; however you have the option to mount the pedestal flush to the base on a 90° angle. Diagram Three Sample Vice Holder The sample vice which is provided with the system can be easily alternated with the pedestal. 1.2cm square mounting blocks (provided with the system) or the other pegs are held by the vice clamp.
EMS-002 Cryo Workstation, 240V
The EMS-002 Rapid Immersion Freezer allows for reliable, virtually routine freezing of biological samples. The EMS-002 achieves precision ultra-rapid cooling by quenching the sample in a cryogen, cooled to near liquid nitrogen temperature, using a stainless steel vacuum insulated Dewar and a copper liquefaction chamber. Applications: Cryofixation of virus partials, isolated or assembled macromolecules, emulsions, paints and polymers and suspensions and tissues. Cryo-techniques such as freeze fracture/etch, freeze drying, freeze substitution, CryoSEM and CryoTEM. Easy Setup and Operation Liquid nitrogen is poured into the Dewar. The initial cooling of the cryogen liquefaction chamber takes about fifteen to twenty minutes. Gaseous cryogen (usually propane) is then added to the liquefaction chamber slowly enough to allow it to condense and fill the chamber with liquefied cryogen. The cryogen is allowed to cool to near liquid nitrogen temperature supervised by the user-configured digital temperature controller. The EMS-002 Cryo Workstation requires only about ten minutes to cool to operating temperature after the first filling of cryogen, and less than five minutes after subsequent fillings. The rate of cooling can be controlled by adjusting the level of liquid nitrogen. Heaters, built into the chamber walls and actuated by a temperature controller, evenly warm the cryogen above its freezing point. Sample Preparation Cryo TEM The sample can be prepared using any size EM grid. Once the sample is applied to the EM grid, the excess must be carefully blotted away. The goal is to form a thin meniscus of specimen across the holes in the grid, then insert the specimen holder in the Drop Anvil of the EMS-002. Freeze Fracture and Freeze Substitution Use a panama hat-shaped specimen carrier. Place a thin spacer, typically an EM slot grid, on the top of the sample hat, and a second hat upside down on the sample, to prevent the sample from being crushed. If the specimen is large and sturdy enough, a hole can be made in one or both hats to allow the cryogen to directly contact the specimen. Results and Techniques Approximately 100 meters per second is the optimal plunging speed and provides more efficient heat exchange. The freezing is fast enough to capture rapid events and labile structures that are not seen in chemically fixed material. The technique provides higher rates of cooling than can be attained by plunging the sample into the cryogen by hand, because of the high velocity and accuracy of the sample entering the cryogen. Fast Recycling The recycling time of the EMS-002 Cryo workstation is only a few minutes, just long enough to be ready again when processing of the last sample is finished. The device will operate for about two hours on ten litres of liquid nitrogen, including the initial cool down. Alternative Cryogens Commercial grade propane is the most commonly used cryogen, for which the temperature is set for -185°C. Chemically pure propane (map. 190°C) can also be used, but it is significantly more expensive than commercial grade propane. A non-flammable cryogen, SUVA-124 (by Dupont), and even Ethane can also be used as cryogens in the EMS-002 Cryo Workstation. NOTE: The EMS-002 should be operated in a fume hood when using flammable cryogens.
EMS7000smz and EMS5000mz Vibrating Microtomes
EMS7000smz and EMS5000mz Our top-of-the-range high precision, vibrating microtomes, are the finest slicers in the world for all specimen preparation. The EMS5000mz is a very competitively priced high precision unit with a z-axis deflection of only 1-2 microns and a blade advance controllable to 10 microns/sec. The EMS7000smz unit has a Sub-Micron z-axis deflection and a blade advance controllable to 10 microns/second. On both units the section thickness step size is 0.001mm and each vibrating microtome is supplied with its own z-axis calibration verifier. All types of sectioning is possible including sectioning for visual patching of neurological tissue, heart, and lung, and much more. The effect of excessive Z-axis deflection on the health and viability of the tissue preparation has been much discussed since the publication of Jonas et al (2003) and the EMS7000smz will now deliver perfect sections every time with sub-micron Z axis deflection across a wide range of vibration speeds and amplitudes.The affordable 5000mz offers almost the same features with Z-axis deflection of 1-2 microns at all vibration speeds and amplitudes, which is less than most other microtomes on the market. The all-new user interface is both easy to use and versatile. The 7000smz and the 5000mz offer similar operation at the press of a button. The versatility includes simple operation at the push of a button or a range of changeable and programmable parameters and a menu enables the settings of your own preferences. The EMS7000smz also gives you longevity of performance, in other words it not only gives submicron performance out-of-the-box but the advance vibrating mechanism does not contain bearings and other components subject to wear. Consequently it will retain the sub-micron Z-axis deflection performance for years to come, giving you consistency in your biological preparation. Tissue cooling is accomplished by the use of either an ice-water bath or an electronically controlled thermo-electric cooler. Other options include a LED cold light source and a magnifier or a stereoscope for clear observation whilst slicing. Features at a Glance: Includes a Z-axis devification device Z-axis blade adjust minimiser Custom blade holder with angle set to user requirement Set START and STOP position for blade travel Vibration speeds from 50 to 120 Hz Amplitudes from 0.5mm to 2.25mm Controlled blade advance at 10 microns per sec. Ice water bath easily removed for cleaning Optional LED light source Optional magnifier for clear observation Optional stereoscope, choice of x5-x10 fixed or x10-40 zoom for optimal observation On the EMS7000smz additional features: Choice of manual or automatic operation Auto programming by storage of the first slicing speed and distance profile Model EMS7000smz EMS5000mz Section thickness step size mm 0.001 0.001 mm Z-Axis Deflection Measured at all speeds and amplitudes with capacitance devices traceable to National Standards Sub-micron 1-2 microns Blade oscillation frequency at calibration 25 Hz 25 Hz Blade oscillation amplitude at calibration 2.25mm 2.0mm Total travel of Bath table 19mm 19mm Bath table rise & fall speed 0.85mm/sec maximum 0.85mm/sec maximum Cutting head advance speed when slicing From a minimum of -1.00 mm/sec, through the zero point to a maximum of 1.00 mm/sec From a minimum of -1.00 mm/sec, through the zero point to a maximum of 1.00 mm/sec Cutting head advance speed resolution 0.01mm/sec 0.01mm/sec Cutting head advance speed max (fast mode) /- 4.0mm/sec /- 4.0mm/sec Cutting head retraction speed 4.0mm/sec 4.0mm/sec Blade oscillation frequency (amplitude dependent) 50Hz to120Hz 50Hz to 100Hz Frequency step size 5Hz 5Hz Blade oscillation amplitude (nominal) 0.5mm to 2.5mm 0.5mm to 2.0mm Blade oscillation amplitude step size: 0.25mm (nominal) 0.25mm (nominal) Machine Dimensions LxWxH (With magnifier and cold light options but without scope) 390 x 390 x 260mm 390 x 390 x 260mm Tissue bath and tissue mount Tissue bath is demountable for easy cleaning. Tissue bath is demountable for easy cleaning. Power requirements:(selectable) 115VAC 60Hz or 230VAC 50Hz 115VAC 60Hz or 230VAC 50Hz Power rating tba tba Fuse rating (115V) (230V) (115V)(230V) Weight, lift handles supplied 43 Kg (excluding microscope) 43 Kg (excluding microscope) Shipping weight 58 Kg (excluding microscope) 58 Kg (excluding microscope) Shipping dimensions in wooden crate 475mm x 540mm x 840mm 475mm x 540mm x 840mm Options Temperature Controlled Standard Tissue Bath Model 7610 The EMS 7610 Tissue Bath Cooler Unit is intended for use with our EMS 7000 and EMS 5000 range of vibrating microtomes. Unfixed brain slices sectioned at 4°C give better tissue preservation and are viable for longer in-vitro recordings. Additionally, some enzyme histochemical techniques give better staining results when sectioned at low temperatures. The EMS 7610 Series coolers use "Peltier" thermoelectric elements. The stainless steel tissue bath and mount are detachable to allow sterilisation by autoclave if required. The equipment comprises of a mains operated control unit, cooling element, tissue bath and specimen holder. The control unit houses a power supply and temperature control circuitry. The cooling element assembly consists of thermoelectric "Peltier" elements, temperature feedback sensors and a cold water fed heat exchanger. Current from the power supply flows through the thermoelectric elements, which act as heat transfer units. Heat is drawn off, cooling the solution in the tissue bath. The heat generated by this process is removed by the water supply fed through the heat exchanger. The unit uses a proportional temperature control algorithm to maintain temperature stability. This will hold the bath temperature to within 0.5°C of the temperature set point. There will, however, be a small variation in temperature vertically through the bath. Experience will show the best temperature to be set for any given requirement and ambient temperature. Product Specifications: Display Resolution: 0.1°C Temperature Accuracy: /- .5°C *Temperature Range: 8°C to 0°C* Voltage Requirements: 230V 50Hz or 115V 60Hz Power Rating: 60W Inlet Fuse Rating: 2A (*Note that the actual temperatures achievable will be dependent upon the solutions used and local temperature conditions) 2. Integrally Mounted Cold Light Source Model 7000-2-1 To facilitate the careful slicing operation, it is most important that the progress of the blade through the tissue be clearly observed. This observation is used to ensure control of the speed of advance and of the oscillation of the blade. Two elements are required for clear observation, light and magnification. Product Features: Ability to direct the light where needed with two flexible fiber optic light guides. Maintains temperature stability of the tissue or surrounding a.c.s.f. Light Intensity is adjustable by a rotary dial knob. 20W/12V Halogen lamp 2000 hour life approx. Fan and electronic power supply Adjustable with potentiometer Twin 500mm fibre optic light guides 3. Integrally Mounted Magnifying Glass Model 7000-1-3 For general global slices a magnifying glass offering approx 2x magnification is sufficient. However, if specific loci are under study then an inspection microscope is desirable. The binocular inspection microscope with 10x-40x magnification, a zoom of 1x-4x and a working distance of 80mm is ideal for the task. Product Features: 115mm Diameter 305mm Focal Length 2x Magnification 4. Integrally Mounted Inspection Microscope (10x-40x) Model 7000-1-1 5. Integrally Mounted Inspection Microscope (x5 and x10) Model 7000-1-2 Consumables 1. Ceramic Blades Model 7550-1-C Product Description The new blade holder design is now included with all Integraslice and Vibroslice instruments. EMS 7550-1-C and EMS 7550-1-SS are designed for new Integraslice instruments. Product Features: Made from ultra hard zirconium Honed by a process of "lapping" both sides of the single bevel to micron flatness Great rigidity, hence a straight cutting edge Prolonged slice life, especially in the most difficult of tissues such as young brain or very old brain Inert and impervious to corrosion Initial higher cost is offset by its longevity Fits into a special blade holder now standard on both Integraslice and Vibroslice instruments Dimensions: 38mm x 7mm x 0.5mm 2. Stainless Steel Blades Model 7550-1-SS Double beveled on both faces, honed to an acute cutting edge and hardened to 56 Rockwell. Nevertheless, stainless steel is relatively soft and these blades are usually used once or at a maximum changed every day. These blades are for use in specially designed blade holders for Vibroslice instruments manufactured prior to 2003. Blade Specifications EMS 7550-1-SS EMS 7550-1-C Material Stainless Steel Ceramic Dimensions 38mm x 9mm x 0.5mm 38mm x 7mm x 0.5mm Geometry Double sided Single sided Bevel Double bevel Single bevel Honing Ground edge Lapped edge Packaging 50 per pack 5 per pack Order Infromation EMS7000smz and EMS5000mz Vibrating Microtomes, Options, and Accessories The finest vibration microtomes with sub-micron z-axis deflection at all speeds and amplitudes of vibration EMS 5000-mz Vibrating Microtome, includes demountable tissue bath and sample blades in stainless steel and ceramic. 115V or 220V each EMS 7000-smz Programable Vibrating Microtome, includes demountable tissue bath and sample blades in stainless steel and ceramic plus z-axis calibration device. 115V or 230 V each Optional Accessories: EMS 7610 Standard Temp. Controlled Tissue Bath each EMS 7000-1-3 Magnifying Glass, mounted to machine each EMS 7000-1-2 Inspection Microscope fixed x5 & x10 each EMS 7000-1-1 Inspection Microscope zoom x10-40 each EMS 7000-2-1 Cold Light Source each Spares and Accessories: EMS 7000-3-1 Tissue Bath, Ice bath and specimen mount complete set EMS 7000-3-2 Tissue Bath, Ice bath and tilting specimen mount complete set EMS 7000-3-5 Tissue Bath, Ice bath, in perspex to fit previous Campden model 752 tissue mount set EMS 7000-4-1 Specimen Mount Only each EMS 7000-4-2 Tilting Tissue Mount each EMS 7000-5-1 Blade holder- standard each EMS 7000-5-3 Blade holder- special angle each EMS 7000-6-1 'Opti-Cal' Z-axis measurement, traceable to national standards each EMS 7000-7-1 Blade handling tool each EMS 7000-50-1 Tool kit each EMS 7000-60-1 Transit crate each EMS 7550-1-SS Stainless steel blades Pack/50 EMS 7550-1-C Ceramic blades Pack/5
Germinator 500, Dry Steriliser
The GERMINATOR 500 is a simple, inexpensive way to quickly decontaminate your micro-dissecting instruments between procedures. This method is effective for quickly decontaminating micro-dissecting tools. The unique stainless steel glass bead bath remains at a constant 260ºC, allowing you to insert the tips of your instruments whenever you wish to decontaminate them. And it’s fast, most micro-dissecting instruments decontaminate within 15 seconds. No messy chemicals and no need for open flames in the lab. Features: Easy to use Fast, effective decontamination Small footprint saves bench space Proven durable design Perfect for micro-dissecting scissors and forceps U.L. Listed 1 YEAR WARRANTY Modular Cord Exclusive Well Collar TECHNICAL DATA Measurements: Unit: 171.45 x 133.35 x 133.35mm (H x W x D) Stainless Steel Well: 50.8mm diameter x 101mm deep (inside dimensions) Weight: 2.05kg Electrical: 120V, 110W, 0.75A (continuous operation) U.L. Listed Detachable Power Cord Operating Temp: 233ºC Glass Beads: 1.5mm lead free glass balls USER MANUAL The Germinator 500 is designed to decontaminate metal micro-dissecting instruments only. It is to be used exclusively for research purposes. The Germinator 500 should not be used as a substitute for traditional methods of terminal sterilisation. Effective sterilisation cannot be assured due to lack of routine sterilisation-efficacy monitoring methods for glass bead sterilisation. The Germinator 500 has been designed and built to pass the Validation of Dry Steriliser Spore Suspension Test: USP XXIII, Part 1211. The Germinator 500 consists of a stainless steel well filled with tiny glass beads and heated to 260°C for dry heat decontamination. Heat from the glass beads decontaminates the surface of the instrument in contact with the beads. Small instrument tips such as scissors, forceps, scalpels and tweezers are decontaminated in about 15 seconds; larger instruments may take as long as one minute. The GERMINATOR 500 can remain turned on all day without overheating or emitting an unpleasant odour. Larger instruments easily fit in the 2-inch diameter x 4-inch deep stainless steel well. Maintenance The Germinator 500 requires little maintenance. It is recommended that the unit be cleaned periodically and the glass beads changed as recommended. Inspection Inspect the Germinator 500 before each use. Make sure the unit is on a stable surface prior to use. Inspect the power supply cord and all the visible components for damage, and have the unit serviced if necessary. Be sure the glass beads are within 1/2” of the well rim. If low, replace the beads as instructed above. Cleaning Unplug the unit and let it cool prior to cleaning. Never immerse the GERMINATOR 500 in water. Clean exterior with a damp cloth and mild soap. Do not use aggressive detergents on the exterior. The stainless steel well can be cleaned with a mild disinfecting detergent and a damp cloth. Never remove the cover to the unit. Service information With proper use your Germinator 500 may not need service for at least five years. The expected lifetime of the unit is ten years from the first date of operation. For all service and repairs, contact us at 1-800-523-5874. How to store unit Turn power switch off and allow the unit to cool down for one hour. Unplug the power cord from the wall outlet. Detach the cord from the back of the unit. When cool, pour the glass beads into a container and close the container. Wipe the interior of the well and the top of the unit with isopropyl alcohol. Allow the disinfected unit to dry before placing in a storage area. Do not lay the unit on its side. Verification of performance It is recommended that the operator periodically check the operating temperature of the unit. Use a thermometer that will exceed 288°C. Insert the probe into the centre of the bead mass. If the unit is operating below 182°C, contact Electron Microscopy Sciences Customer Service department as soon as possible. WARNING! DO NOT USE MERCURY-FILLED GLASS THERMOMETERS Proper use of beads It is important to stir the beads regularly to reduce bacteriological risk. If the unit is allowed to sit uninterrupted for more than thirty minutes—the operator should stir the beads before inserting instruments for decontamination. This will equalise the temperature of the beads and prevent the beads on top from losing too much heat. The glass beads should last about six months under normal use. Inserting dry instruments that are free of tissue and other culture will help extend the life of the glass beads and your instruments. CAUTION! TO REDUCE THE CHANCE OF INJURY, CLEAN GLASS BEADS SPILLAGE IMMEDIATELY. Special markings Refer to the following warnings, safety precautions, and operating instructions.
Haematospin 1300 Haematocrit Centrifuge
THIS PRODUCT IS DISCONTINUED BY SUPPLIER TECHNICAL SPECIFICATIONS CLASSIFICATION Class I Equipment SHOCK PROTECTION Type B DEGREE OF MOBILITY Mobile Equipment INGRESS OF LIQUIDS Ordinary MODE OF OPERATION Intermittent POWER CONSUMPTION 380 watts at 220/ 240v 50 Hz. AC. FUSES Mains 2 x 3.15amp MOTOR High precision AC/DC series wound, brush gear, class B insulation, specified to BS5000 pt 2, computer wound coil windings Balanced armature and high speed bearings TIMER Electronic, graduated in 5,10,15,20,30,45 secs, 1 min and all 30 sec intervals up to and including 15, 20, 25 and 30 mins. BRAKE Automatic SPEED 11,800 rpm with 24 place rotor RCF 13,000 g with 24 place rotor NOISE 62 dB running with 24 place rotor WEIGHT 8 kg. net (without rotor) ACCELERATION 0-11,800 rpm in 70 secs, with 24 place rotor DIMENSIONS 23.5cm height x 27cm deep x 26cm wide CONSTRUCTION Steel casing, steel lid FINISH Body Cream white baked enamel Lid Matt black baked enamel
Haematospin 1400 Micro Haematocrit Centrifuge
THIS PRODUCT IS DISCONTINUED BY SUPPLIER TECHNICAL SPECIFICATIONS CLASSIFICATION Class I Equipment SHOCK PROTECTION Type B DEGREE OF MOBILITY Mobile Equipment INGRESS OF LIQUIDS Ordinary MODE OF OPERATION Intermittent POWER CONSUMPTION 380W at 220/240V 50Hz AC FUSES Mains 2 x 3.15amp MOTOR High precision AC/DC series wound, brush gear, class B insulation, specified to BS5000 pt 2, computer wound coil windings Balanced armature and high speed bearings TIMER 0-15 minute timer, graduated in 1 minute increments. Automatic switch at zero PULSE Push button switch - hold to run. BRAKE Automatic SPEED 11,800 rpm with 24 place rotor RCF 13,000 g with 24 place rotor NOISE 62 dB running with 24 place rotor WEIGHT 5.1 kg. net (without rotor) ACCELERATION 0-11,800 rpm in 70 secs, with 24 place rotor DIMENSIONS 23cm height x 27cm diameter CONSTRUCTION Aluminium casing, steel lid FINISH Body Cream white baked enamel Lid Matt black baked enamel
HEPA Filter Kit for Diaphragm Vacuum Pump
HEPA Filter Kit for Diaphragm Vacuum Pump Comes with labels How to Change HEPA Filters: Unscrew the adjust knob. (FV‐W‐110/240 Only) Remove the labels. (Top Cover Only) Unscrew the screws. Open the cover. Change the filter. Close the cover. Screw the screws. Attach the new labels. (Top Cover Only) Change intervals vary depending on ambient conditions.
LabGlass High Purity Water Stills
Why you should choose a Water Still from ProSciTech: ProSciTech can ship stills direct from the manufacturer and at lowest prices. If the electrical supply is compatible, we can supply these stills advantageously to Europe, America, Japan and elsewhere. These stills are made by a leading manufacturer and employ top technology and innovative designs. AND we provide old‐fashioned service ‐ including any glass replacement parts for 25 year‐old stills. Stills provide the cheapest source of high purity water for all applications. Stills have no expensive consumables to replace on a regular basis. Stills handle poor water supply quality, when other systems would clog. Stills remove bacterial contaminants. Stills are simple to use and maintain. Stills are "immune" to obsolescence due to continued parts supplies. CHOOSING A WATER STILL: Is double distilled water required? If yes, then choose the DELTA for 3L/h double distilled output. The Cascade dual‐feed, when fed with a supply of single distilled water will put out double distilled water too. What production quantity of single distilled water is required? 6 litres per hour ‐ choose the DELTA 3 litres per hour ‐ choose the CASCADE If you have changing needs, and could use all of the above configurations from time to time, choose the DELTA. This still can be configured by you to provide the quantity and quality of water you need, when you need it. DESIGN AND PRODUCTION OF STILLS: Attractively finished in a durable colour powder coating. Each still may be wall, shelf or bench mounted and operated to maintain a full reservoir, with still operation controlled in accordance with distillate usage. All models have a feed water supply failure protection (a pressure switch set to 12 p.s.i.; lower pressure switches are available) This feature is combined with over‐temperature switches for each boiler to allow safe 24 hour unattended operation. The CASCADE produces single distilled water at 3 litres per hour. For that, cooling water at the rate of 90 litres/hour is required. This often is from mains water, which could be stored in a holding tank for irrigation or recirculating cooling system are available. The use of those system requires a dual feed unit. The DELTA, unlike other stills available, can be reconfigured at any time by the user in a matter of minutes to produce single distilled water at 6 litres per hour, or double distilled water at 3 litres per hour. Both models, the CASCADE and DELTA, are also available for dual‐feed. Dual‐feed uses mains (or refrigerated and recirculated) water to cool the condensers. A second, usually low‐pressure water line from a tank of pre‐treated water (this may be rainwater, filtered, single distilled or deionised) is the feed for distillation. All boiler parts are common to all models. This includes the condensers, boilers, heating elements, element sheath, sealing washers, heat and pressure switches. There is one exception: the Delta's first distillation stage employs slightly larger elements to ensure that boiler two cannot run dry. Shipping dimensions and weights: Carton Size ‐ all models 76cm x 45cm x 53cm Packed weight: CASCADE 17kg, Dual Feed 18kg; DELTA 19kg, Dual Feed 20kg. DISTILLATE QUALITY pH 5 to 6.5 approx. Conductivity of single distilled is 2.0 µS/cm Pre‐treated feed water and or double distilled is 1.5 µS/cm and is pyrogen free. Distillate quality may vary depending on the quality of the water source. The pH and conductivity of distilled water changes when exposed to the atmosphere. This is a result of carbon dioxide absorption that is temperature dependent, and is not an indication of a change in overall purity.
Laboratory Microwave Oven, EMS 820
Testimonial Leydon, 19th April 1994 The EMS 820 is a user friendly microwave oven for the pathology and histology laboratory, receiving praise from the people using it. It is much easier to program than the H2500, currently made by EBS, and mentioned in the Microwave Cookbook for Microscopists (Kok and Boon). When we wrote the mentioned cookbook, the EMS 820 was not yet available, therefore we could not give a judgement of its practicality for the histology laboratory. A.E. Boon Reasons for using laboratory microwave ovens: Literally hundreds of laboratory procedures using microwave ovens have now been published including procedures such as heating, fixation, staining, polymerisation, decalcification and immunology. Microwaves allow for accelerated procedures in almost each and every step of sample preparation. Not only does microwave technology speed up reaction times (even more important is that it improves the retention of soluble antigens and preserves antigen immunoreactivity better than conventional fixation methods). KITCHEN VERSUS LABORATORY: In short, microwaves have now become a standard piece of equipment found in most microscopy labs. Many people feel a standard kitchen oven is good enough and heck if it cooks popcorn just think what it can do to my precious tissue... This is not the case. Commercially available units, better known as kitchen ovens, were not designed to offer any type of consistency, which is necessary in the lab. The electronics are variable at best, there are numerous hot and cold spots in the oven but most problematic is the function and how the unit operates changes as the electronics and samples warm up. Hence these commercially available units offer inconsistent results and grave disappointment with results when one tries to duplicate the results of fellow scientists using a different commercial oven then his fellow scientists‐ This is why laboratory microwave ovens were developed ‐ to eliminate the guesswork and offer consistent results from use to use and scientist to scientist. Laboratory ovens, such as the EMS 820 meet strict clinical standards. They offer reproducible results. They do not damage the sample and they promote the safest handling of dangerous chemicals and infectious agents. THE MICROWAVE ADVANTAGE: Faster fixation Higher quality preservation Faster staining Faster polymerisation Antigen retrieval Faster decalcification Rapid drying, etc SAFETY: Microwaves are electromagnetic waves similar to radiowaves. They have relatively low energy so the myths of "glowing in the dark" are ridiculous. To set the record straight: DO'S: Use a leak detector Inspect and clean door seals, frames and hinges Disinfect top surfaces Oven should be vented to a fume hood Use microwave transparent containers Use open containers Operate the oven with minimum liquid volume Use mitts when handling hot containers DON'TS: No food in the oven which is used for lab No entry of wires or metals Do not use caustic agents Do not use flammable solvents Do not use containers with metal parts Do not irradiate closed containers Microwave ovens rarely leak but monitoring them regularly is advisable for precaution. For protection from leakage these points should be stressed: Test the unit frequently Increase your distance from the running unit Minimise your exposure time, use shortest procedures possible WHAT NOT TO DO: Never operate an empty oven longer than one minute Rapid on/off: do not cycle the magnetron on and off by rabidly opening and closing the door Do not use auto on programming whereby your unit turns on automatically when the door is closed Do not place or run away wires in the oven Do not use flammable chemicals (greater than 50% concentration)
LYNX II Automated Tissue Processor
LYNX II is designed to be superior to the Lynx Tissue Processor, with many improvements along with the ability to handle larger size specimens for Histology. The Lynx II Automated Tissue Processor for Histology and Microscopy is a cutting-edge tissue processor that can work with any plastic resin or paraffin wax. The Lynx II has an ability to hold 24 reagent vials for EM processing. HP (Histology processing) is optional and can be achieved with 12 larger size reagent vials. Lynx II has two independently operated heating/cooling stations in both EM and HP modes. LYNX Tissue Processor Setup Operational Overview of Lynx II Lynx II is a tissue processing system that operates in batch mode. Tissues may be prepared for EM or HP. Both types of tissues are handled in the same way: The sample arm is loaded with samples, and the reagent carousel is loaded with reagents. The Lynx II rotates the reagent carousel depending on the software chosen by the user until the selected reagent station is at the designated location. The specimen is immersed in the reagent at the processing station. Based on the agitation and vacuum settings, the Lynx II periodically moves the sample arm to provide agitation and applies vacuum when agitation is not active. When the timer in a given reagent runs out, the sample is lifted and the processing station is rotated to the next reagent. By using the program numbers, the user will pick the processing for EM or HP. Programs 1–10 are for EM processing, and programs 11–20 are for HP processing. In addition to selecting the correct program number, it is the end user's duty to use the correct carousel positions and the appropriate form of reagent vials depending on the choice of EM vs. HP processing. After properly configuring the machine for EM or HP processing, the operator loads the reagent carousel and samples, selects the desired program, and begins the run. The sample is stored in the last reagent at the end of the run before the user removes it. Features 12 reagent vials for HP processing, each 125mL volume 24 reagent vials for EM processing, each 25mL volume 10 Programs for EM and 10 programs for Histology Two independently controlled heating/cooling stations Easy replacement of reagent carousel Paraffin processing Sample agitation Vacuum infiltration during processing Choice of plastic and metal containers for improved chemical compatibility and heat transfer Optional external media based interface to a PC for ease of programming and allowing for the editing of programs on a PC and the transfer of programs between the Lynx II and the PC Built-in fume extraction and ability to connect the discharge hose to lab exhaust system Internal battery back-up during power fail to protect the samples Ability to continue run after power interruption User Friendly Operator Interface Optional external UPS for continued processing during longer power outages Functional Specifications System Configuration 1. Lynx II is designed with the following functional modules. Rotary mechanism to rotate the reagents EM and HP reagent carousel Up/Down mechanism to move the sample in and out of the reagent as well as to provide the agitation EM and HP Sample arms Tight seal for all reagent containers except for the one at the processing station. Vacuum pump and a pressure sensor or a switch Two Peltier operated heating/cooling station with independent temperature sensors and controls Fume extraction fan and air flow sensor 2. Reagent Carousel A reagent carousel, either a 24-position one for EM reagents or a 12-position one for HP reagents, can be mounted. The EM carousel accommodates tubular vials that are approximately 30.4mm in diameter and 63.5mm wide, while HP vials are approximately 55.8mm in diameter (to accommodate 44.4mm x 30.4mm cassette flat) and 63.5mm tall. The diameter of the carousel is approximately 304.8mm. For easy handling, each vial has a flange. Plastic and aluminum vials are also available. For some types of manufacturing, the vials on a given carousel can be mixed and balanced between metal and plastic vials. Before starting a run, the Lynx II has a built-in sensor for detecting if the correct reagent vial types (EM vs. HP) are mounted on the device. It is sufficient to be able to detect only one of the two vial varieties. 3. Sample Arm The sample arm is equipped with either an EM sample holder mechanism or an HP sample holder mechanism. Each sample holder mechanism is intended to be mounted on the arm at a position that will center the samples in their respective reagent containers. To fit various samples, the top of the Sample Arm can be modified. 4. Agitation and Vacuum If the sample is in the reagent tube, the arm provides gentle agitation (approximately 5 mm up and down travel at a rate of 2.5 mm per second or one up/down agitation per second). Five agitation strokes are performed in 5 seconds and are performed every 45 seconds. If vacuum infiltration is chosen, agitation will be applied for 5 seconds, followed by 40 seconds of vacuum. These agitation-only or agitation-vacuum cycles will be continued for the remainder of the station period. A connection on the sample holder assembly allows you to connect the vacuum hose from the vacuum pump. 5. Heating-Cooling Stations The Lynx II has two separate and similar heating/cooling stations (HCS). Each HCS will have its own temperature sensor and is heated or cooled by a Peltier module. One HCS is at the sample processing station, and the other is one place ahead of it near the processing station. This arrangement enables reagent pre-heating or pre-cooling for a program in which the reagents are organised sequentially and contiguously. Each HCS can be set to a temperature ranging from 4 to 65 degrees Celsius. The actual temperature would be accurate to 2 degrees Celsius when measured on the surface of the Heating – Cooling station. The set point at the HCS surface is achieved in 7 minutes after the reagent arrives at the station. 6. Fume Control The Lynx II comes with a fume extraction fan that directs fumes from the reagent/ sample area to an exhaust outlet. An external pipe may be attached to the device and routed to a fume hood or laboratory exhaust system. The fume extraction system keeps fumes clear from electronics and mechanism parts. 7. Lid/Cover The fume system includes an acrylic cover with a latch. The system's sensor will detect whether or not the cover is latched. A run cannot begin (and will pause) if the cover is not locked. However, there is a software override that allows the device to run without locking the cover. 8. Seal for the Reagent Vials Except for the one underneath the processing station, all reagent vials have a tight seal. Reagents Used During the processing, the materials used in the various components and subsystems of the Lynx II are compatible with the reagents mentioned below. HP Processing Formalin Alcohol Xylene Xylene Substitutes Water Paraffin 10% bleach solution (to wipe clean the system) EM Processing Buffers Osmium Tetroxide Ethyl Alcohol Acetone Propylene Oxide Epoxy/acrylic resins 9. Operation During Power Outage To protect the sample during a power failure, the Lynx II has a small internal battery (capable of shifting the sample from up to down position and moving the carousel to the next reagent place at least three times without being re-charged). When the Lynx II's sensors detect that the A/C power is about to fail, it will switch off the power to the Peltier elements at both heating/cooling stations. It will then ensure that the samples are placed in a reagent vial. If the samples are not in a reagent vial (because Lynx II was about to move the carousel towards the next reagent location or was in the center of the rotation), the carousel will be moved forward by one position, and the samples will be lowered into the reagent at the processing station. The time the power failure alert was triggered will be registered, and the device will enter standby mode. Lynx II can restart the run once power is restored and sustained for at least 30 seconds. As Lynx II restarts, the station timer will not restart until the heating/cooling stations have met the appropriate temperatures. If the temperature at the processing station or the previous station was above 55 degrees Celsius during an HP cycle, the station timer does not start for an extra 60 minutes. This function ensures that the paraffin at each of the two stations fully melts before going on to the next station. Specifications: Dimensions: Max Width: 22.5" Max Depth: 19.0" Max Height: 12.0" (cover closed) Max Weight 50lb (32 kg) Electrical Design: Display: 8 lines x 20 column text only LCD Keypad: Membrane style keypad with tactile feed back Mechanism Drive: Stepper Motors Power Requirements: 100 - 115 volts AC, 50-60 Hz, 8 Amps Max 230 volts AC, 50-60 Hz, 4 Amps Max Back-up power: NiCd Battery, 12 volts, 1 Amp-hour Fuse Rating: 100 – 115 volt version 8 Amps x 250 Volts AC, Time Delayed (Slo-Blo) 230 volt version 5 Amps x 250 Volts AC, Time Delayed (Slo-Blo) Operating Environmental Conditions: Ambient Temperature: 20 to 32 o C (70 to 90 deg. F) Relative Humidity: 20-80% non-condensing Ambient Pressure: 28"-32" (70-80 mm) of Hg Storage/Transportation Conditions: Temperature: 10-40 deg. C (50 to 102 deg. F) Humidity: 15%-80% non-condensing Pressure: 28"-32" (70-80 mm) of Hg Lynx II accessories and spare parts L12610 Histology Kit (Histo Sample Holder, Histo Sample Arm, Histo Carousel, Power Cord, Operator Manual, Histo Aluminium Containers (24), Histo Plastic Containers (36), Plastic Caps (36), Exhaust Hose, Small Cassettes (100), 2 Sample Record Note Pads, Spare Fuses. L12612 Electron Microscopy Kit (EM Sample Holder, EM Sample Arm, EM Carousel, Loading Jig, Power Cord, Operator Manual, Aluminium Containers (24), Plastic Containers (72), Plastic Caps (72), Exhaust Hose, Small Baskets (100), Large Baskets (100), Basket Lids (100), 2 Sample Record Note Pads, Spare Fuses. L12655 EM Reagent Vials, Bag of 72 L12656 EM Regent Vial Cap, Bag of 72 L12657 HP Reagent Vials, Bag of 36 L12658 HP Reagent Vial Cap, Bag of 36 L12659 EM Aluminium Container (Box of 24) L12660 HP Aluminium Container (Box of 12) L13154 EM Sample Holder Assembly (Basket Stem Assembly) L12663 HP Cassette Holder Assembly L12661 Vacuum Pump Tubing (1 each) L12662 Vial Seal Assembly L12995 EM Sample Arm Seal L12996 HP Sample Arm Seal L12952 EM Carousel (Without Aluminium Containers) L12953 HP Carousel (Without Aluminium Containers) L12664 Replacement Back-up Battery L12665 Lynx II Operator Manual L12667 Lynx II Service Manual The Lynx II for Microscopy comes with: EM Sample Holder, EM Sample Arm, EM Carousel, Loading Jig, Power Cord, Operator Manual, Aluminium Containers (24), Plastic Containers (72), Plastic Caps (72), Exhaust Hose, Small Baskets (100), Large Baskets (100), Basket Lids (100), 2 Sample Record Note Pads, Spare Fuses. The Lynx II for Histology comes with: Histo Sample Holder, Histo Sample Arm, Histo Carousel, Power Cord, Operator Manual, Histo Aluminium Containers (24), Histo Plastic Containers (36), Plastic Caps (36), Exhaust Hose, Small Cassettes (100), 2 Sample Record Note Pads, Spare Fuses. The Lynx II for Microscopy and Histology comes with everything found in both units. All of the above accessories may be purchased separate or in the kit.
Mini Vacuum Desiccator
EMS71246-01 Our Mini Vacuum Desiccator consists of a clear top section, a white bottom section, an O-ring and a vacuum retention valve. This model is suitable for the protection of organic and inorganic samples from contaminants and oxidation and for the storage of calibration samples. The desiccator has a conical connecting piece, that can be removed after the evacuation. Therefore, an unintentional venting is prevented, which is also ideal for transport of samples. The evacuated volume allows recording of up to 18 scanning samples (Standard Fei / Philips) or some grid boxes for transmission electron microscopy. INSTRUCTIONS Working with shielding gases: For venting of the desiccator screw the vacuum connection (in the recipient) to the valve. Remove the outer O-ring. The space is required for the U-shaped auxiliary tool. Pull the valve in the arrow direction until it stops, and fix the tube clip with the U-shaped auxiliary tool. Attach the aluminium bolt to the desiccator housing and adjust the stopper in such a way that the shielding gas can still easily flow (about 0.2 mm between bolt and glass). Remove the U-shaped auxiliary tool and press the tube clip into the desiccator. The tube clip can be removed by turning counter clockwise until the inner ring is free (maintain contact pressure to the recipient). The tube clip can now be removed. For the transport of samples or when traveling, we recommend the desiccator not be filled with shielding gas, but to keep it under vacuum.
PELCO® BioWave® Pro+ FAQ
How do I achieve wattages below 250W? Full instructions on changing the wattage are in the section 'Wattage Adjustment/Calibration' of the manual. It is a straightforward procedure which gives the operator a good feel for the flexibility of the power output of the unit. What is the function of the temperature probe? In the past, before variable wattage and the development of the ColdSpot (a recirculated waterbed for the surface of the microwave cavity), sample temperature control was required from the temperature probe (known as setting a temperature restriction), otherwise the sample would in all likelihood overheat. The temperature probe does not report accurate temperatures in small fluid volumes (below 20mL). Due to this problem the protocol design for EM processing does not use the temperature probe, but variable wattage and the use of the ColdSpot ensure sample temperature control for the times and wattages given. The ColdSpot has a port to hold the temperature probe during microwave processing. Use the probe to check sample temperature after a processing step is completed and adjust time or wattage if you feel the sample temperature is too high or low. Resin polymerisation is controlled by the power output chosen (wattage) and the presence of the ColdSpot. Low wattage at the beginning ensures a slow temperature rise of the water. High wattage at the end produces the heat necessary to complete the polymerisation. Do I need a water load? No additional water load (beaker) is required and one should not be added when the ColdSpot is present. How do I maintain ColdSpot temperature? That is indirectly controlled automatically by the microwave circuitry. The ColdSpot will warm gradually with continuous microwave operation and will reach a maximum of approximately 50°C during the polymerisation process at high wattage. At much lower wattage the ColdSpot temperature will be at about 40°C. What's the best sample holder to use? The Prep-Eze can be used for all the processing steps: fixation, dehydration and resin infiltration. The holder can be cleaned easily with acetone following resin infiltration.
PELCO® Dimpler™ Tool Repadding
The polishing cloth on PELCO® Dimpler™ Tools eventually wears out or becomes contaminated; the replacement padding kit makes it simple to mount new cloth on the PELCO® Dimpler™ Tools. The Repadding Tool Kit includes (shown on the left in Figure 1): Aluminium Mandrel, a replaceable Glass Slide Slide Holder Base The Mandrel fits the arbor on the Tool, while the Base and Slide provide a clean, stable surface on which to roll the adhesive cloth of the Repadding Strips onto the Tools. The pre-cut cloth strips are supplied separately. The Repadding Strips are available for all of the padded PELCO® Dimpler™ Tool sizes, including the 2iM 1/8” wide Standard Tool and the 1/8”, 1/4”, and 1/2” wide Large Area Tools. The various Tool sizes and several Repadding Strips are shown below in Figure 1. Figure 1: Repadding Tool Kit shown with PELCO® Dimpler™ Tools and Available Repadding Strips APPLICATION 1) Each set of Repadding Strips is adhesive-backed. When preparing to repad a Tool, ensure the Tool core is clean and oil-free, then remove one of the pre-cut cloth strips from the sheet of 10 as shown in Figure 2 below. 2) Each Repadding Strip has a white backing attached to it as shown in Figure 3. To start, peel the edge of this backing away from the cloth. 3) Then, take the end of the cloth and align it with the tool as shown in Figure 4. 4) Next, firmly press down and begin to rotate the Tool in one hand while peeling the cloth away from the backing with the other (Figure 5). While applying the cloth to the Tool, it is important to maintain the correct tension on the cloth such that when ends meet, the cloth edges almost touch. Figure 2: Repadding Strips and an Unpadded 1/2” Large Area Tool Core Figure 3: Separating the Backing from the Repadding Strip Figure 4: Aligning the Repadding Strip onto the Tool Figure 5: Wrapping the Cloth around the Tool 5) Then press these ends down/together onto the tool (Figure 6). The cloth will relax slightly over time, and getting the tension correct ensures that gap at the joint is minimal. A small gap has been found to be acceptable and does not impede sample processing. Figure 6: Joining the Cloth around the Tool 6) Finally, roll the tool under firm pressure against the glass slide as shown in Figure 7. This will ensure that the thickness of the Repadding Strip is even and there are no air bubbles. In the case that a gap opens at the edges of the Repadding Strip as the adhesive relaxes, press the ends together and roll the tool again to close the gap and ensure sticking of the Strip. Figure 7: Rolling the Tool to Even Out the Padding and Activate the Pressure-Sensitive Adhesive
PELCO® easiGlow™ Glow Discharge System
Glow Discharge Methods supported: Surface Condition Charge Atmosphere Hydrophilic Negative Air Hydrophilic Positive Air (with subsequent magnesium Acetate treatment) Hydrophobic Positive Alkylamine Hydrophobic Negative Methanol Efficient glow discharge performance for the PELCO easiGlow™ with optimum pressure at given distance from sample to glow discharge head. For those labs which need both hydrophilic and hydrophobic surface treatments on a regular base, a dual glow discharge system is available to avoid cross contamination of the glow discharge chambers. Hydrophobic surfaces are preferable when organic solvents are used. Since the optimised vacuum level for glow discharge procedures is in the range of 0.45 to 1.1 mbar, we offer the affordable GD7 dual stage rotary vacuum pump designed for this pressure range. Advantages of the PELCO easiGlow™ system: Precise and easy vacuum setting Short cycle times Consistent results Auto, programmed and manual mode Intuitive touch screen for display and operation Supports hydrophilic/hydrophobic and negative/positive modes Affordable, compact, standalone system L‐Gasket protects glass jar at base GD7 Rotary Vacuum Specifications Pumping speed 8.4m3/hr, 140 l/min, 5 CFM @ 60Hz 7.0m3/hr, 117 l/min, 4.2 CFM @ 50Hz Ultimate vacuum 7 x 10‐3 MBAR (5 x 10‐3 TORR, 5 Micron) Vacuum connection KF16 Exhaust connection Compatible with 1/4" NPT Thread Oil capacity 500cc Operating temperature 7 ‐ 40°C (45 ‐ 104°F) Dimensions 380 x 150 x 320mm 15" x 6" x 12.5" (H x W x L) Weight 14kg / 33.5 lbs. Power rating 110 ‐ 115V 60 Hz, 7.0A 220 ‐ 250V, 50/60 Hz, 4.3A Specifications of the PELCO easiGlow™ Glow Discharge system Plasma Current 0‐30 mA HV Power supply 30W Continuous Rating Glow Discharge Head Polarity User Selectable positive or negative Process Platform ?75mm with recess for 25 x 75mm slide Glow Discharge Platform Height Control 1‐25mm Preprocess Hold Timer 0‐14411 sec. Process Timer 0‐900 sec. Chamber Size Diameter 20mm x 100mm H with sealed Delrin top plate Process/Vent gas inlets 2 each x 6mm barbed, individually user selectable for process, purge or vent High Voltage Vacuum Interlocks Dual hardware and software interlocked Vacuum Control Pirani Gauge, ranging from atmosphere to 0.01 mbar Working Vacuum Range 1.1 ‐ 0.20 mbar for optimal glow System Control / Display 3" Touch Panel with LED backlight and 5 function keys Operation Modes Auto Programmed (4 user programmed protocols) Manual/Diagnostics Data Logging Capabilities Step #, Vacuum (mbar), Current (mA), Voltage (V), and polarity, once per second Instrument Size 305 L x 292 D x 230mm H Weight 6.2kg GD7 Pump (optional) 380 L x 150 D x 320mm H Weight 12.2kg Laboratory Footprint with optional pump 381 W x 292 D x 330mm H Power Requirements 120V 60Hz, 15A or 230V 50Hz, 10A Service Instrument Power Rating 100‐240VAC 60/50Hz 60W Plus Pump, IEC Inlet Optional Pump Power Rating 120/230V 60/50Hz 250 W (1/3 hp) Vacuum Pump Minimum requirements 2.9 m3/hr, 48 l/m, 1.73 CFM @ 60Hz 0.30 mbar Ultimate Vacuum 2.5 m3/hr, 41 l/m, 1.65 CFM @ 50Hz 0.30 mbar Ultimate Vacuum Vacuum Pump Power Outlet on Instrument 8A max IEC Outlet Vacuum Inlet KF 16 flange Data Logging Capabilities Step #, Vacuum (mbar), Current (mA), Voltage (V), and polarity, once per second Communication Port USB Outlet
PELCO® EM Pro Vacuum Chamber
The PELCO® EM Pro Vacuum Chamber is designed for use inside all PELCO BioWave® series microwave processors. The chamber is made of chemically resistant, microwave transparent polypropylene and designed for vacuums down to 20” of Hg and temperatures up to 70°C. Figure 1. Chamber components Top Plate (A) and Base Unit (B) form the chamber with the L-gasket (C). The Base Unit is fitted with a ¼" Barb Fitting (D) to attach the vacuum hose. The L-gasket is secured by a Cable Strap (E). The ¼" vacuum hose with PTFE O-rings secure the hose to the vacuum chamber and to the internal vacuum port in the microwave cavity. Optional Top Plate with Temperature Probe Port for Vacuum Chamber also available. Supplied with two caps—one solid and one with opening for temperature probe. Installation and Use of Vacuum Chamber: The appropriate installation of the vacuum chamber is shown below. See Figure 2. The recommended vacuum level is 20” of Hg (508 Torr). The PELCO BioWave® series of microwaves have a preset vacuum to this level along with a venting feature within the protocols. Vacuum grease is not required for the operation of this chamber. Always make certain the top is seated correctly when the vacuum is initiated. The PELCO® EM Pro Vacuum Chamber is recommended for assisting in chemical fixation and resin infiltration. Any other applications should be carefully researched and tested prior to incorporation into a protocol. Figure 2. Installation WARNING: The use of vacuum with solvents is not recommended. For processing steps where water is present, temperature restrictions below 70° must be used. For processing steps where formalin is present, temperature restrictions below 40° must be used. Liquids with these characteristics will boil and be drawn into the vacuum system and severely damage the components of the microwave. Instructions for replacing cable strap for L-gasket: Place L-gasket on vacuum chamber by stretching the gasket past the edge and gently “walking” the gasket around the edge of the chamber. Once the gasket is in place, place the cable strap over the gasket and thread through the lock. See Figure 3. Use a pair of needle nose pliers and grip the end of the strap. Turn the pliers as if you are rolling the excess strap. Continue this motion until the cable strap is tight around the gasket and chamber. See Figure 4. Cut off the excess strap. See Figure 5. Use a file to smooth off the cut end at the lock. See Figure 6.
PID Controller Operating Instructions, Model DCB10
Operating Instructions for Digital Control Box - Model DCB10 Description: Designed for use with hotplates and digestion blocks requiring an external control due to hazardous substances. The powder coated steel chassis houses a Digital PID Temperature controller, Solid State Relay with HeatSilnk and Power Switch OverTemp and heating indicator and sockets for temperature probe and supply cord. Operating Instructions: Plug Hotplate into 3 pin socket on back of control box and the sensor into the 2 pin socket, before turning on power. The Controller will not function without the sensor plugged in. Ensure the sensor is fully inserted into the sensor port located on the cord entry side of the Hotplate casing. Set Controller to required temperature and enter. Unit will now be heating, indicated by Red Indicator next to Controller. The maximum set temperature (SV) has been limited to 280°c by the high limit parameter (SU-H). Should a faulty relay allow the hotplate temperature to rise above 300°c the alarm function of the Temptron will inhibit heating. The "Alarm" indicator will illuminate and stay on until the hotplate temperature (PV) drops below the alarm value (ElHd). This can be set to any temperature below 300°c. To reset the alarm - turn power switch off, then on again. Sensor Compensation (PU-b) is adjusted for accuracy at 100°c. It is typically -10°C which willl cause a discrepancy at ambient of 10°c (i.e. if ambient = 24, PV = 14°). This is due to the sensor being located very close to element to minimise temperature fluctuation. The Temperature Controller has an AutoTune Function to give best heat up characteristics. Should you encounter any problems with overshoot or slow response under load, consult the manual for instructions on AutoTuning. Parts List: 1 x Shimaden digital PID controller - Part No. SR1-8P 1 x 60 cm x 30 cm Teflon Coated Aluminium Hotplate - Part No. 207T 2 x 1200W Elements for 207T hotplate - Part No. VG9701 1 x Solid State Relay 40 AMP - Part No. SSR40DC 1 x Power Relay - Part No. 737-1X 1 x Heatsink - Part No. HO560 1 x Alarm Relay c/w base - Part No. SCL-DPDT 1 x 10Amp 3 pin socket - Part No. CLI415L 1 x 6mm SS sheath type K thermocouple for Shimaden digital PID controller - Part No. 200K2000 1 x Miniature plug and panel mount jack 1 x Indicator Switch - Part No. C5403FQ 2 x Indicators - Part No. C0430FQ 1 x Stainless Steel Baffle - Part No. 207DSB 6 x 12mm Pillars - Part No. CS12 1 x Digital Control Box (10 AMP) - Part No. DCB10 1 x Filter - Part No. NFM210S Solid State Heat Controller Please inquire for pricing support@proscitech.com Operating Instructions Description Designed for use with hotplate units or flask heaters up to 2400 watts, this unit features a solid state power regulator which varies the voltage to the heater element from approximately 30 volts up to 230 volts. The constant supply gives a very stable temperature control with virtually no fluctuation in process temperature, as opposed to switching type heat controls such as simmerstats which can give fluctuations of 30° or 40°C in element temperature. The temperature dial has a 0 to 100% scale divided into 2% increments. Also available in 15amp and 20amp models. Instructions Plug flask heater or Hotplate into the 3 pin socket on the front of control unit and plug control unit cord into 10 amp power outlet. Turn on power switch and adjust dial to required rate of heat.
Precision Pulsed Laboratory Microwave Oven
Note: This based off the EMS Laboratory Microwave Oven, E97100 The Precision Pulsed Laboratory Microwave Oven is a state of the art solution for performing many of the steps in sample preparation prior to either light or electron microscopy. Procedures such as fixation, staining, dehydration, decalcification, impregnation and immunohistochemistry are possible in this instrument, with processing times dramatically reduced and improved results. For optimum performance, the oven features a well defined adjustable effect, an accurate temperature control, a well ventilated chamber, and three timing modes for the operation of the unit. Our temperature control not only prevents the tissue in the chamber from becoming denatured (by high temperature) but it also assists in pulsing the microwave effect in small precise portions. The temperature control has a direct effect on the magnetron (the microwave generator) as it allows the unit to switch on and off in the most efficient way at the preset effect level. The set effect is adjustable at any time either before or during a run. This aids in the balance of the microwave load and the content of the microwave absorber. It should be noted that the penetration rate and process times are influenced by the presence of microwave radiation in the chamber and for this reason it is imperative that the effect rate is adjustable at any time during a run. Inside the chamber there is a manifold for up to 5 tubes, this allows bubble mixing in 1 to 5 containers at once. There is a built in air pump to facilitate mixing and this avoids temperature gradients. These adjustable bubble mixers make this instrument ideal for decalcification. The EMS Microwave Oven is most versatile. It provides a choice of three optional operating modes: Continuous mode for processes longer than 1 hour 40 minutes Timer controlled processes up to 1 hour 40 minutes Short run processes less than 6 minutes The oven chamber is made entirely of stainless steel, no solutions are absorbed into it and so it is easy to clean. The floor of the unit, which covers the rotator cavity, is a glass fibre reinforced silicone plate which serves as a thermal insulator and does not absorb microwaves. The plate is highly resistant to chemicals. This instrument is designed for easy operation and maintenance. All of the controls can be set with one hand and are easily accessible allowing for changes at any time. An adjustable thermal probe encased in a white Teflon holder (a microwave transparent material) comes with the unit. The probe can be set at different heights to accommodate all different types of containers and volumes of liquid. Attached to the probe is an adaptor to hold one of the mixing tubes; this is important because the liquid which is regulated by the thermostat needs to be mixed well to avoid temperature gradients. The probe automatically measures the existing temperature and displays it. The unit has a built in reflector inside the chamber which evenly distributes the microwaves. A rotator is built into the unit, as well, to prevent "Hot‐Spots" from forming even for very small volumes (e.g. reagent drops on glass slides). FEATURES Easy operation and maintenance Easily adjusted effect % knob Accurate temperature control Well ventilated chamber 3 different timer modes Built in air pump for mixing Manifold for up to 5 mixing tubes Adjustable temperature probe holder Stainless steel chamber Glass fibre reinforced silicone floor On/off rotator switch Built in reflector for even distribution of microwaves SPECIFICATIONS Magnetron Effect 780W Effect Range 20‐100% Temperature Range 25‐100°C Temperature Accuracy +/‐ 0.5°C within 25‐70°C Temperature Variation +/‐ 0.4°C at 40°C Balanced effect with 100mL H?O load and 100mL H?O Sample Timer 1 sec ‐ 99min 99sec Continuous Run Timer override‐works as a count up timer for an indefinity period of time Safety Switch Off At 105°C chamber temperature Measurements (Inner) 345 x 350 x 225mm Outer Dimensions 600 x 470 x 530mm Warranty One Year The temperature probe of all makes of laboratory microwave ovens are not covered by warranty. These probes are expensive but have a very long life ‐ except when abused. The face of the current model EMS 820 Microwave Oven DIFFERENT TIMER OPTIONS Continuous Run: For long processes requiring more than 1 hour 40 minutes. (e.g.: decalcification) Timer Controlled Run: For processes less than 1 hour 40 minutes. (e.g.: section staining, fixation, etc.) Short Runs: For very short processes, usually shorter than 6 minutes, without the need for the thermal probe. (e.g.: immunohistochemistry) ADVANTAGES Ease of Use: When using a laboratory microwave oven, one has to determine the set effect to use depending on the type of sample, temperature, and load. Our unit has the effect control knob easily accessible at all times, and can be changed even during the middle of a run. The effect percentage setting is easy to read at all times. Continuous Run Mode: For longer procedures (greater than 99 minutes 99 seconds) our unit runs continuously ‐ the timer works in a count up mode for an indefinity period of time. There is no need to shut off the unit and begin again. Multiple Bubble Mixing: A 5‐port manifold for mixing up to 5 different containers at once. Stainless Steel Compartment: Very easy to clean and resistant to staining or scratching. Resistant to all staining solutions. Adjustable Temperature Probe Holder: It will accommodate any size container and it is flexible and very easy to align. Glass Fiber Reinforced Silicone Floor: It comes standard with the unit and it covers the rotator when it is not in use. Ventilation: Our unit has a built in powerful fan which accommodates at least 2 meters of tubing.
QCoat v QSeries sputter coaters
Quorum Technologies are delighted to announce our new generation of EM coaters, available to order from February 2025. The range includes a new Rotary and Turbo-backed system, available in three configurations tailored to meet various application needs. Designed for versatility and exceptional ease of use, offering automated control for Electron Microscopy sample preparation. From February, the new range will be available for order, while the existing QSeries range will be discontinued. All configurations of the QSeries range (Q150R, Q150T, Q150V) will be supported for seven years from the last shipment date. The new range not only matches the capabilities of the QSeries but also offers enhanced functionality, ensuring continuous product improvement and maintaining high standards. Introducing the new QCoat Range The range includes a new Rotary and Turbo-backed system, available in three configurations tailored to meet various application needs. Designed for versatility and exceptional ease of use, offering automated control for Electron Microscopy sample preparation. Feature Tilt stage as standard allows for better coating deposition and coverage of samples. Tilt stage as standard allows for better coating deposition and coverage of samples. FTM as standard allows control of coating, providing better precision and control. FTM as standard allows control of coating, providing better precision and control. Allows for better viewing and avoids screen glare. New tilt touchscreen allows for better viewing and avoids screen glare. Allows for faster pump-down, optimal vacuum of 1x10-6mbar. Better vacuum measurement Allows for faster pump-down, optimal vacuum of 6x10-3mbar. Removable chamber allows for the chamber to be easily removed when switching between processes. No needs to unbolt and adjust. Removable chamber allows for the chamber to be easily removed when switching between processes. No needs to unbolt and adjust. - Upgraded stainless steel hose Allows for Pt to be sputtered as standard Features Recommended for low-medium magnification Suitable for use with Tungsten/LaB⁶ SEM and Benchtop SEM Rapid cycle time for maximum sample throughput Tilt stage design giving uniform coating of complex 3D structures Film Thickness Monitor (FTM) to control deposition of coating Intuitive and responsive touchscreen colour panel design Multi-colour LED visual status indicator offering process updates Pre-set recipes for standard protocols resulting in sample reproducibility Customizable recipes for tailored applications Easy and quick set-up of profiles Multiple stage options based on application needs Specification QSeries - Q150R QCoat – RotaQ Chamber Instrument size with chamber open: 440mm (W) x 860mm (H) x 551mm (D) Borosilicate glass 150mm diameter x 127mm (H) ES version included short and extended chamber. UPGRADED Borosilicate glass 150mm diameter x 220mm (H) ES version includes an extended chamber. Screen Multi-colour LED visual status indicator touch screen. UPGRADED Touch screen with tilt movement. Vacuum 5x10-6mbar UPGRADED 6x10-3mbar FTM Not included. UPGRADED Included as standard. Stage Standard 6 place stub stage 50mm Ø rotation stage with rotation speed of 8-20 rpm. UPGRADED Tilt stage with 6 place 50mm Ø rotation stage with rotation speed of 8-20 rpm. Target/Source Disc-style 57mm Ø. 0.1 mm thick gold (Au) target Carbon fibre woven cord 1m is supplied with carbon fibre head as standard. Disc-style 57mm Ø. 0.1mm thick gold (Au) target Carbon fibre woven cord 1m is supplied with carbon fibre head as standard. Stage options Rota Cota, 4’ Wafer stage, Slide stage. Rota Cota, 4’ Wafer stage, Slide stage. Head options Sputter head, carbon rod, metal evaporation and glow discharge head. Sputter head, carbon rod, metal evaporation and glow discharge head. Target options Au, Au/Pd, Ag, Pt Au, Au/Pd, Ag, Pt Features High vacuum instrument for ultra-high-resolution EM Ultra fine grain size for high-resolution analysis Rapid cycle time for maximum sample throughput Tilt stage design giving uniform coating of complex 3D structures Film Thickness Monitor (FTM) to control deposition of coating Intuitive and responsive touchscreen colour panel design Multi-colour LED visual status indicator offering process updates Pre-set recipes for standard protocols resulting in sample reproducibility Customisable recipes for tailored applications Easy and quick set-up of profiles Multiple stage options based on application needs Specification QSeries - Q150 T & V QCoat – TurboQ Chamber Instrument size with chamber open: 440mm (W) x 860 mm (H) x 551mm (D) Borosilicate glass 150mm diameter x 127mm (H) ES version included short and extended chamber. UPGRADED Borosilicate glass 150mm diameter x 220mm (H) ES version includes an extended chamber. One Chamber only. Screen Multi-colour LED visual status indicator touch screen UPGRADED Touch screen with tilt movement Vacuum Q150 T 5x10-5mbar & Q150 V 1X10-6 mbar UPGRADED 1x10-6mbar FTM Not included UPGRADED Included as standard Stage Standard 6 place stub stage 50mm Ø rotation stage with rotation speed of 8-20 rpm. UPGRADED Tilt stage with 6 place 50mm Ø rotation stage with rotation speed of 8-20 rpm. Target/Source Disc-style 57mm Ø, 0.1mm thick gold (Cr) target Carbon fibre woven cord 1m is supplied with carbon fibre head as standard. Disc-style 57mm Ø, 0.1mm thick gold (Cr) target Carbon fibre woven cord 1m is supplied with carbon fibre head as standard. Stage options Rota Cota, 4’ Wafer stage, Slide stage. Rota Cota, 4’ Wafer stage, Slide stage. Head options Sputter head, carbon rod, metal evaporation and glow discharge head Sputter head, carbon rod, metal evaporation and glow discharge head Target options Pt, Ir, Au, W, Au/Pd, Cu, Ag, Ti, Co, Sn, ITO, Mg, Ta, Fe, Al, Mo, Ni Pt, Ir, Au, W, Au/Pd, Cu, Ag, Ti, Co, Sn, ITO, Mg, Ta, Fe, Al, Mo, Ni
Retriever Slide Stainer
The unique retriever solves the problem of staining formalin fixed tissues. This is a low-cost solution to all known significant difficulties with immunohistochemistry on paraffin slices. The combination of ease of use and great consistency of findings will provide you with the highest quality immunostaining. The Retriever is a bench-top model for thermally processing slides of formalin-fixed, paraffin embedded tissues prior to immunostaining. The model has been designed to ensure identical processing of all the samples during a processing cycle, as well as the identical processing of the samples in individual sessions. The retriever preserves processed tissues. Features: Run antigen unmasking in 6 various buffers at once. Perform gentle antigen retrieval that does not damage the tissue morphology. Get identical results every time. Compare a series of slides treated in independent sessions. Full set contains the processing unit, slide chamber (allows to fit 18 Slides) and Chamber Rack for 6 Slide Chambers (108 slides in total). How does the Retriever work? Ok, basically it is a pressure cooker. However, a pressure cooker specially designed to unmask the antigen on tissue sections. The core principle is heating of the chambers with the slides at high temperature (>120°C) and high pressure. Sensors control the heating profile for the temperature and pressure to be reached at certain pace and over certain time. We did a lot of tests to find the optimal settings. When the required temperature is reached, it will be kept for several minutes. After that the slides will cool over 2 hours. Specially designed thermal walls of the unit control the speed of cooling of the inner chamber and slides. Who would benefit from using our Retriever? Investigative Pathology, where the high quality of staining (a picture may be published!) is required. Any lab that is short on technical personnel: any student or post-doc can process slides for the staining without using much time Small routine pathology labs, where a limited number of slides should be processed daily Anyone who uses highly valuable samples, such as tissue arrays or unique tissue samples: Simplicity and reliability of the unit ensures the safety of your sample, and a high quality antigen unmasking How To Use: Step 1. Deparaffinized sections on slides are placed into the Processing (Tissue Slide) Chambers (EMS62705-01). Retriever can accommodate simultaneously from 1 to 6 Chambers, which allows you to process a series of slides in up to 6 different antigen unmasking buffers within the same session. Fill the chambers with a buffer of your choice. Step 2. Place the Chambers into the Rack. Fill the Retriever with 750 ml of deionized/distilled water. Place the Rack into Retriever. Close the lid with a simple twist. Step 3. Push the Start button. The tissues will be processed automatically. In about two hours (depends on the load) you can open the lid and proceed with immunostaining. Technical Specifications of Retriever: Height335 mm Capacity 9 litres Max. Instrument length 228 mm Width 340 mm Net Weight 4.5 kilos Internal chamber Dimensions (d/h) 210/230 mm Max. Load Weight 3.0kg Other Specifications: Fuses - Located under the control module, fuses F1 OA, 32 x 6.3mm, ceramic sand filled, Mains plug top fuse (User replaceable), F1 3A to BS1362 UK ONLY. Rating - Models are rated continuously for intermittent use. Body- Deep drawn aluminium. Lid - Aluminium. Heater- Externally surface mounted mechanically fixed electric element. Temperature Cut Out - Thermal fuse. Pressure - Calibrated pressure release valve. Max. Single Fault Temperature - 133.3°C Environment Conditions - indoor use - temperature 5°C to 40°C - altitude up to 2000m - maximum relative humidity 80% for temperatures up to 31°C decreasing linearly to 50% relative humidity at 40°C. - mains supply voltage fluctuations not to exceed +10% of the nominal voltage Input Connections - Mains inlet socket 'hot' format conforming to IEC 302. Safety Shut Down - See "Temperature Cut Out". Choosing A Buffer: If you already know the buffer that can be used for Microwave treatments of sections in order to unmask you antigen of interest, the high chances are that the same buffer may be used in Retriever. To improve the morphology of tissue of the processed sections, use one of our supplied ready-to-use buffers by choosing them according to pH. We have commercially available the following buffers: R-Buffer A pH 6.0 R-Buffer B pH 8.0 R-Buffer C pH 4.5 R-Buffer U pH 6.0 G variants of the same buffers for a more gentle processing of the tissues. To successfully retrieve the antigen of interest on fixed sections, please remember that two factors define the choice of buffer: The nature of the antigen/epitope and of the antibody used for its detection The fixative used and the degree of fixation. Correction for Fixative & Degree of fixation The suggested protocol for processing tissues is optimized for routinely formalin-fixed and paraffin embedded material. If the tissue used for sections was insufficiently fixed, overfixed (was left in formalin for too long), or other fixative was used, the protocol may require some modifications. The easiest correction is to use EMS's own specially formulated buffers for a gentle (G) tissue processing. Use them instead of the basic buffers. For overfixed material try using U buffer or run the additional cycle in the same buffer. Fixative Used on Tissues Buffer Formalin, bufferedR-Buffer A, B, C or U Formalin-Zn R-Buffer A, B, C or U Mirsky fixative Under testing Boonfix I Under testing Boonfix II Under testing PLP R-buffer AG, BG, or CG Choosing Buffer: Correction for the Nature of the Antigen and Epitope. The choice of the buffer depends on the nature of the antigen and the location of the epitope of interest. We advise you to first run the test for the most appropriate buffer using the tissues where the expression of the antigen does occur. General guidelines for choosing the buffer: Most of the nuclear antigens (apoptosis-related, survival-related, proliferation-related) R: Buffer A (or AG). Cell adhesion molecules, cell membrane antigens (extracellular domain) R: Buffer A (or AG) Cytoskeleton and cytoskeleton-associated molecules - R:Buffer A (or AG) Intracellular domain of some adhesion molecules and surface receptors - R:Buffer B (or BG) Intracellular domain of some adhesion molecules and surface receptors - R:Buffer C (or CG) Most of antibodies raised against a linear peptide - R: Buffer U (or UG) Publications involving examples of the retriever in various types of research and clinical studies: Cancer Pathology Urology. 2006 Sep;68(3):578-82. Inactivation of myopodin expression associated with prostate cancer relapse. Yu YP, Tseng GC, Luo JH. Acta Oncologica. 2008;47(8):1539-45. Aberrant expression of cyclin E in low-risk node negative breast cancer. Ahlin C, Gruhne B, Holmqvist M, Zetterberg A, Fjällskog ML. Breast Cancer. 2010 Jul;122(2):315-24. Numb protein expression correlates with a basal-like phenotype and cancer stem cell markers in primary breast cancer. Rennstam K, McMichael N, Berglund P, Honeth G, Hegardt C, Rydén L, Luts L, Bendahl PO, Hedenfalk I. Pancreas. 2009 Apr;38(3):259-66. Neurogenin 3 and neurogenic differentiation 1 are retained in the cytoplasm of multiple endocrine neoplasia type 1 islet and pancreatic endocrine tumor cells. Lejonklou MH, Edfeldt K, Johansson TA, Stålberg P, Skogseid B. Hormone and Metabolic Research. 2008 May;40(5):354-9. Lack of nuclear expression of hairy and enhancer of split-1 (HES1) in pancreatic endocrine tumors. Johansson T, Lejonklou MH, Ekeblad S, Stålberg P, Skogseid B. Leukemia and Lymphoma. 2010 Jul;51(7):1269-77. Potential biomarkers of bortezomib activity in mantle cell lymphoma from the phase 2 PINNACLE trial. Goy A, Bernstein SH, McDonald A, Pickard MD, Shi H, Fleming MD, Bryant B, Trepicchio W, Fisher RI, Boral AL, Mulligan G. Clinical Cancer Research. 2006 Jul 1;12(13):4080-9. PHA-680632, a novel Aurora kinase inhibitor with potent antitumoral activity. Soncini C, Carpinelli P, Gianellini L, Fancelli D, Vianello P, Rusconi L, Storici P, Zugnoni P, Pesenti E, Croci V, Ceruti R, Giorgini ML, Cappella P, Ballinari D, Sola F, Varasi M, Bravo R, Moll J. Cell Biology Molecular Biology of the Cell Vol. 20, 2572-2581, May 15, 2009. Regulation of Mammary Gland Branching Morphogenesis by EphA2 Receptor Tyrosine Kinase. David Vaught, Jin Chen, and Dana M. Brantley-Sieders. Molecular Cancer Therapeutics. 2008 Mar;7(3):590-8. Dual inhibition of VEGFR and EGFR signaling reduces the incidence and size of intestinal adenomas in Apc(Min/+) mice. Alferez D, Wilkinson RW, Watkins J, Poulsom R, Mandir N, Wedge SR, Pyrah IT, Smith NR, Jackson L, Ryan AJ, Goodlad RA. Molecular Cell BiologyMol Cell Biol. 2004 Apr;24(7):2673-81. Inhibition of Mist1 homodimer formation induces pancreatic acinar-to-ductal metaplasia. Zhu L, Tran T, Rukstalis JM, Sun P, Damsz B, Konieczny SF. PNAS U S A. 2007 Jun 12;104(24):10063-8. Loss of a quiescent niche but not follicle stem cells in the absence of bone morphogenetic protein signaling. Kobielak K, Stokes N, de la Cruz J, Polak L, Fuchs E. Science. 2010 Jun 4;328(5983):1278-8. Meiotic recombination provokes functional activation of the p53 regulatory network. Lu WJ, Chapo J, Roig I, Abrams JM. Cancer Research. 2006 Jan 1;66(1):242-7. Mist1-KrasG12D knock-in mice develop mixed differentiation metastatic exocrine pancreatic carcinoma and hepatocellular carcinoma. Tuveson DA, Zhu L, Gopinathan A, Willis NA, Kachatrian L, Grochow R, Pin CL, Mitin NY, Taparowsky EJ, Gimotty PA, Hruban RH, Jacks T, Konieczny SF. JCI. 2008 Jan;118(1):89-99. Glypican-1 modulates the angiogenic and metastatic potential of human and mouse cancer cells. Aikawa T, Whipple CA, Lopez ME, Gunn J, Young A, Lander AD, Korc M. Cancer ResearchCancer Res. 2007 Jan 1;67(1):276-80. Transgenic overexpression of RasGRP1 in mouse epidermis results in spontaneous tumors of the skin. Oki-Idouchi CE, Lorenzo PS. Dermatology Journal of Investigative Dermatology. (2005) 124, 615-621 Mutations in Gasdermin 3 Cause Aberrant Differentiation of the Hair Follicle and Sebaceous Gland. Declan P Lunny, Erica Weed, Patrick M Nolan, Andreas Marquardt, Martin Augustin and Rebecca M Porter Immunology, Research & Clinical Journal of Experimental Medicine. 2009 Aug 3;206(8):1661-71. Loss of the lupus autoantigen Ro52/Trim21 induces tissue inflammation and systemic autoimmunity by disregulating the IL-23-Th17 pathway. Espinosa A, Dardalhon V, Brauner S, Ambrosi A, Higgs R, Quintana FJ, Sjöstrand M, Eloranta ML, Ní Gabhann J, Winqvist O, Sundelin B, Jefferies CA, Rozell B, Kuchroo VK, Wahren-Herlenius M. Bone Marrow Transplantation. 2008 Dec;42(12):807-18.GVHD after chemotherapy conditioning in allogeneic transplanted mice. Sadeghi B, Aghdami N, Hassan Z, Forouzanfar M, Rozell B, Abedi-Valugerdi M, Hassan M. Journal of Reproductive Immunology. 2010 Mar;84(2):176-85. Pro-inflammatory and anti-inflammatory cytokines in human preterm and term cervical ripening. Dubicke A, Fransson E, Centini G, Andersson E, Byström B, Malmström A, Petraglia F, Sverremark-Ekström E, Ekman-Ordeberg G. Neuroscience Journal of Comparitive Neurology. 2008 Sep 1;510(1):79-99. Distinct molecular pathways for development of telencephalic interneuron subtypes revealed through analysis of Lhx6 mutants. Zhao Y, Flandin P, Long JE, Cuesta MD, Westphal H, Rubenstein JL. Reproductive Biology Fertility & Sterility. Vol 91, Issue 4, Supplement, Pages 1420-1423 (April 2009) Expressions of steroid receptors and Ki67 in first-trimester decidua and chorionic villi exposed to levonorgestrel used for emergency contraception Chun-Xia Meng, M.D.ab, Li-Nan Cheng, M.D.b, Parameswaran Grace Luther Lalitkumar, Ph.D.a, Lin Zhang, M.D.b, Hui-Juan Zhang, M.D.b, Kristina Gemzell-Danielsson, M.D., Ph.D. Human Reproduction. 2010 Apr;25(4):874-83. Effects of oral and vaginal administration of levonorgestrel emergency contraception on markers of endometrial receptivity. Meng CX, Marions L, Byström B, Gemzell-Danielsson K. Virology & Microbiology Virology. 2009 Oct 25;393(2):338-45. An early 'classical' swine H1N1 influenza virus shows similar pathogenicity to the 1918 pandemic virus in ferrets and mice. Memoli MJ, Tumpey TM, Jagger BW, Dugan VG, Sheng ZM, Qi L, Kash JC, Taubenberger JK. mBio 2010 1(1):e00067-10. doi:10.1128 The PB2-E627K mutation attenuates viruses containing the 2009 H1N1 influenza pandemic polymerase. Jagger, B. W., M. J. Memoli, Z.-M. Sheng, L. Qi, R. J. Hrabal, et al. OnePLoS One. 2009 Jun 16;4(6):e5916. Infectious prions in pre-clinical deer and transmission of chronic wasting disease solely by environmental exposure. Mathiason CK, Hays SA, Powers J, Hayes-Klug J, Langenberg J, Dahmes SJ, Osborn DA, Miller KV, Warren RJ, Mason GL, Hoover EA. Journal of Virology. 2009 Apr;83(8):3754-61. Role of sialic acid binding specificity of the 1918 influenza virus hemagglutinin protein in virulence and pathogenesis for mice. Qi L, Kash JC, Dugan VG, Wang R, Jin G, Cunningham RE, Taubenberger JK.
Rotary Vane Pump Dimensions
Model 100‐3.5 Vacuum Pump Dimensions Model 200‐7 Vacuum Pump Dimensions Model 400‐14 Vacuum Pump Dimensions
Sensidyne Gilian BDX-II Pump Charger
EMS65241-50 Operation Highlights Charger provides constant current. Charge time - 15 hours. A RED indicator light will be continuously on while charging. After charge cycle is complete, the light changes to GREEN and a trickle current is keeps the battery pack at full charge. A sequence of flashes will detail charger type and the internal code version when power is applied. While connected to USB power, indicators flashes briefly every two seconds as it looks for a battery to be connected. Once the battery has been disconnected, it continues to search for a connection. If battery is disconnected or power removed, charger will restart the charge when reconnected or repowered. Power Requirements Charger receives power from a USB charging port through a micro B connector. A standard USB charging port can supply 500mA which is sufficient for all charging modes. Other power supplies, computer USB power or cell phone chargers may also be used, as long as they supply the 500mA necessary to power the charger. NOTE: If sufficient power is not available from the power source, charging will stop and the red light indicator will flash rapidly. Power Charger Status Indicators Brief Flashing RED (at 2 second intervals) - Powered, waiting for battery to be attached. Fast Flashing RED (off/on once per second) - Error (Low supply voltage or shorted battery). Continuous RED - Charging. Continuous GREEN - Charge complete, trickle charge rate. PERFORMANCE Flow Range 500 – 3000 cc/min Run Time 10 hours at 2 LPM ENVIRONMENTAL Temperature Ranges Operating Temperature -20°C to 45°C Storage Temperature -40°C to 45°C Charging Temperature 5°C to 45°C Humidity Ranges Operating 5-85 %RH, non-condensing Storage 5-98 %RH, non-condensing GENERAL Controls Power Switch – Flow Control Additional Features – See-through external filter housing, with filter monitoring lens: built-in belt clip Dimensions – 90mm (W) x 100mm (H) x 51mm (D) Weight – 595g ELECTRICAL Battery Pack – 4.8V, 1.8A hour, sealed. Battery Type – Rechargeable nickel metal hydride Battery Charge Time – 14 – 16 hours Expected Battery Life* – 300-500 charge-recharge cycles or 2.5 years (< 20 hours weekly use) 1.5-2.5 years (20 -39) hours weekly use) 1-1.5 years (40-60 hours weekly use) *Inactivity for extended periods may shorten nickel-cadmium battery life. Battery life estimates are based on proper battery maintenance.
Step-up Slide Warmer, Model XH-2003
EMS71319-250 Features Our Step Up™ Slide Warmer's unique patent pending design helps you save space while still meeting a busy lab's needs. The slide warmer is the ideal choice for scientific research organisations, professional laboratories and high school labs (grades 11 and up). Applications include biological, cytological and histological fields. The Premiere Step Up™ Slide Warmer has many advantages in unique design: Easy to assemble and use. Holds twice as many slides as a flat unit of the same size, which makes retrieving slides easier since the end of the slide is not on the flat surface. Has 4 tiered levels, being able to warm up 40 slides at a time. Anodized black surface provides contrast with samples. Removable tray for easy cleaning. Digital LED temperature display and temperature setting. Easier and safer ability to pick up slides since one end of the slide extends off the tiered surface. Electrophoretic surface coating. Warms up slides by heating the tubes and spreads heat throughout the entire surface. Safety Please read this Technical Data Sheet completely and carefully before using. Injury to users or damage to equipment may result if this equipment is not used correctly or appropriately. To avoid injuries to personnel and/or separated samples inside the unit, do not use the following materials in the centrifuge: Radioactive or toxic materials Strong chemical materials Flammable and/or explosive materials To prevent fire or shock hazard: DO NOT expose this unit to water or moisture. This equipment should be used with AC 110V, 60Hz in the USA or Canada (unless labeled otherwise). Note: the 220V, 50Hz available as CAT. 71319-250. While in the slide warmer is heating up and also in use: Make sure there is at least a 12" clearance on all sides of the warmer. Make sure vents are clear of any obstructions. Do not touch the hot surface to avoid burns and/or injuries. Set Up Please note the following conditions/guidelines for safe operation of the Step Up™ Slide Warmer: Open box and remove Step Up™ Slide Warmer from package. Place Step Up™ Slide Warmer on a flat surface. Flip back legs UP to allow slide warmer to stand. When ready to use, plug AC power cord firmly into back of unit and connect to power supply. Specifications Capacity 40 – 3" x 1" slides Temperature range Room temperature to 90°C ±2°C Power requirements AC 110V 60Hz (CAT. 71319-50) 220V 50Hz (CAT. 71319-250) Time setting range 1 – 240 minutes Overall size 14" x 14" x 4"H Shipping weight 22 lb Net weight 18 lb Precautions Before Operating Please note the following conditions/guidelines for safe operation of the Step Up™ Slide Warmer: Ensure area and unit are clean, dry, and well ventilated. Unit must be on a flat, stable work surface Relative humidity needs to be ≤80% Always use with care Avoid moving or touching unit during operation Use only with parts designed specifically for this model or the accessories provided While unit is on, DO NOT touch surface without proper protection. DO NOT use any item on the slide warmer that could scratch or damage its surface Use only the electrical plug supplied with the product NEVER leave the unit unattended while in use DO NOT store unit in direct sunlight Long hair should be pulled back and secured Do not wear loose clothing or jewelry while operating unit NOTE: Equipment has no serviceable parts. Control Panel Temperature display (in °C) Maximum Temperature requested by user (in °C) On/Heat = Unit is Warming/Hot Alarm = Operating Error Turn ON unit to run program cycle Turn OFF unit Increase/Decrease Temperature/Time Directions for Use Plug in Step Up™ Slide Warmer into a standard 110V (or 220V, if applicable). Turn ON Power switch button on back of unit. The light above the word "ON" (located below "Set Temp LED Screen) will light to indicate unit is working. Temperature Display Box will light up to indicate the actual temperature (upper box) and the Set Temperature (lower box). This confirms the unit is on and operating. Adjust temperature by pushing the SET button one time. The top, larger display will read "SP". Use the Increase/Decrease buttons (∧ / ∨) to adjust temperature to desired setting. Once slide warmer reaches desired set temperature, the heat indicator light will turn off and is holding at temperature. Adjust time by pushing the SET button twice. The top, larger display will read "SR". Use the Increase/Decrease buttons (∧ / ∨) to adjust time setting to run for desired amount of time. Once set time is determined, unit will begin to heat until predetermined time is up. Two "beeps" will signal that the Step Up™ Slide Warmer will completely shut off. NOTE: To run unit continuously, set time to "0".
Ultrasonic Cleaner, EBC200M
Blind Cleaning: Ultrasound may be used for cleaning vertical and venetian blinds. Timber blinds may not like the water based cleaning solutions and we advise against cleaning these in ultrasonic cleaners. Clean any spots first with suitable spot cleaner preparations. Blind cleaning tanks are dual tanks, one with ultrasound, timer and perhaps heaters. The second tank is used for water rinsing after ultrasound cleaning. Strips of blinds are cleaned fully immersed. Longer blind strips may be folded, but cleaning is more efficient if they are not folded. Lie the strips flat and clean up to a dozen at a time. If very dirty insert fewer strips. The sonification tank is used with sufficient water to easily cover the blinds with a detergent solution. The commercial cleaning solutions for sonifiers are strong and in large tanks expensive, so many operators use simple detergents. People have their own recipes. Often the detergents are very diluted. Clean for 5 to 20 minutes. Most people buy blind cleaners without heaters. Heated cleaners work faster but they cost more and excessive heat cannot be used. Heated solutions may ruin some blinds, but during cold weather a temperature of 40°C would have some cleaning advantage over cold water. Rinse blinds in water in the second tank, then hang on a rack and dry quickly. To some extent that can be with a fan but most operators use a hairdryer up and down the strip. This is not as slow as it appears since sonification of a second batch would progress during drying time. Forced drying is only required until the strip is near dry. Slow evaporation can lead to water stains near the base of the blades.
UV Mercury Lamps
Obtaining longer UV mercury lamp life: Avoid excessive cold starts. When starting a lamp, the internal pressure is low. During this time, the electrodes are emitting poorly, throwing, or spitting off tungsten into the lamp. This causes points of contamination which can result in premature failure of the lamp. Lamps should be started in high power to shorten the length of time they are in the starting mode. Excessive starts can cause the ends to prematurely darken, resulting in a drop in output as the darkening gradually migrates into the body of the lamp.